Automation-Ready Cell Counting & Analysis Solutions�

Presented By:

Thomas Ehlers, M.S.

Senior Sales Specialist, Cell Imaging & Analysis Technologies

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April 9, 2025

Fit for Purpose Instrumentation

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Cellometer™ Ascend™ �automated cell counter

Cellaca™

high-throughput cell counter and image cytometer

Celigo

whole-well plate-based imaging system for 2D and 3D cell culture

• Fast image capture w/ advanced autofocus
• Cell concentration and viability
• 1-8 samples at a time using disposable counting slides
• 21 CFR Part 11 compatible.

• High throughput cell counting and viability
• 24 samples at a time in 3-6 minutes
• multi-color cell-based assays
• Automation ready
• 21CFR Part 11 compatible

• Whole well imaging of adherent and suspension cells
• Functional live cell assays in any vessel format
• Rapid screening for a variety of cell-level measurements
• Automation ready
• 21CFR Part 11 compatible.

Industry Leaders in Cell Counting & Analysis

CVs of less than 7.5%

The same samples analyzed across our range of cell analysis instruments

Co-developed ISO Guidance and Standards for Cell Counting for Cell Therapies and affiliated content

3 papers co-published outlining methods to assess comparability and bias between orthogonal cell analysis techniques for biotherapeutics

Sarkar, S. et al., Cell & Gene Therapy Insights 2019; 5(1), 117–131, DOI: 10.18609/cgti.2019.016

Stable inter-instrument precision

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40 instruments manufactured over 3 years with 2.5% bias across all samples

Equivalent results across our platforms

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Selected by NIST as the industry expert

Direct Cell Counting w/ AO/PI

• Dual fluorescence viability dye
• One tube, no buffer, wash or extra reagents
• Mix 1:1 with your cell suspension
• No incubation time

Acridine Orange (AO) is permeable to all cells

• AO binds to DNA - fluoresces green

Propidium Iodide (PI) only enters dead cells 

• Binds to Dead Cell DNA - fluoresces red

Quenches the fluorescence of AO

No signal is generated from

non- nucleated cells and debris

Why AO/PI?

AO/PI is less variable than trypan blue

% viable cells predicted

% viable cells measured

Transfusion, 40, 693-696, 2000

Trypan blue is not linear when viability is below 80%

AO/DAPI overestimates cell viability even at 30 mins incubation

AO/PI is more accurate than AO/DAPI

% viable HK cells

Staining time (mins)

J Fluoresc. 2024 Sep;34(5):2263-2278.

Cellaca MX – High Throughput Counting

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Cellaca MX plate with trypan blue to show counting chambers….

Automation Ready

21 CFR Part 11 Compliant Ready

3-6 Min / Plate for 24 Samples

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2.5x magnification

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1.3 µm/pixel resolution

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Detection cell size: 5 - 80 microns

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Linearity: 1.0 x 10⁵ - 1.5 x10⁷ cells / mL concentration

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Volume: 50 to 200 uL (Sample + Dye)

Cell ID and Viability

Flexible Channel Selection: �4 excitation LEDs & 5 emission filters

High-Throughput and Speed

~ 15 seconds per sample

Automated Data Analysis and Report out

21 CFR Part 11 Ready

Automation Ready

Cellaca PLX: HT Cell Counting & Cell Based Assays

Accelerate your research while saving time and costs:

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5.0x magnification

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1.3 µm/pixel resolution

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Detection cell size: 5 - 80 microns

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Linearity: 1.0 x 10⁵ - 1.5 x10⁷ cells/mL

concentration

Cellaca Viability Counting w/ Matrix

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Celigo – Every Cell, Every Well

Celigo is our whole well, live cell screening & analysis platform.

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Automation Ready

21 CFR Part 11 Compliant Ready

Proprietary Optics for High Edge to Edge Contrast

Supports All Culture Vessels

No Trypsinization of Cells Necessary

Label-Free & Fluorescent 2D / 3D Imaging of Adherent & Suspension Cells

Gating Capabilities for Classification and Object Level ID

What Can We Do With Celigo?

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Imaging w/ Celigo

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96 Well Plate Scanning – 5-6 Minutes

Single Cell Detection

Resolution: 1 µm/pixel

10X Magnification

Automatically score and report clonal wells

Monoclonality Reporting for Enhanced Efficiency

Did you know

Automated handling, imaging and reporting has helped developers drive a 10-fold error rate reduction.

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Automation of Platform Use

Confidential | Do not share

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Automation License

• Enables customizable integration of Cellaca or Celigo into a custom robotic workflow.
• Can be quoted with initial purchase or retrofitted to a standalone system.

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Plug-in to Revvity ARS Solutions

• End to end service, with scripts written by Revvity for the customer.
• Customers can pick from a variety of modular components to meet requirements.

Celigo Integration Examples

Expanding Throughput Across Automated Cell Analysis Workflows

Confidential | Do not share

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Cellaca Integration Example

Confidential | Do not share

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Cellaca MX/PLX

Liquid Handler

Plate Hotel

Additional Units

(Incubator, etc.)

Cellaca Workflow Integration: Cell Viability, Concentration, and Cell Characterization

Optimized reagents for cell health and functional assays

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Customizable report templates for automatic generation of graphs, images, charts, & tables

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Incorporate robotics with API compatible instrumentation

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Minimize sample depletion with low loading volume

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Batch samples in 24 well microplates with results in 3 minutes

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Streamlined workflow

Cell Analysis Instrumentation:�Viral Vector Workflow Example

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Viability

Cell seeding

Transduction efficiency

Monitor proliferation

Optimize culture conditions

Characterize protein expression

Culture density and viability

Viral titer, TCID50

Target function and potency

Cell Cultivation & Screening Modular Workstation

Confidential | Do not share

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• Save hours by automating routine processes

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• Support 24x7 operations with improved reproducibility

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• Custom platform supporting integration of application specific instruments

Other Scalable Automation Solutions from Revvity

Confidential | Do not share

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Instrumentation Recap

Thank you! Questions?

Thomas Ehlers, M.S.

Senior Sales Specialist

thomas.ehlers@revvity.com

Revvity.com