RR9 bulk RNA-seq (10 weeks)

GSE242982 Reference scRNA-seq

GSE134873 Aging rod photoreceptor bulk RNA-seq (3M, 12M, 18M, 24M)

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Deconvolution analysis - CIBERSORTx

Estimated cell type proportions (~50% Rod photoreceptors)

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Data integration & normalization

ComBat-Seq: Batch effect correction

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PCA: Quality control & sample clustering

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DEG analysis & overlap: Only 27 shared DEGs

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Next steps: WGCNA: Identify co-expression modules in RR9 and aging dataset

Deconvolution of RR9 dataset

ComBat batch correction of RR9 and aging dataset raw counts and DESeq normalization

Number of DEGs in RR9 and aging datasets

Overlapping upregulated genes: 12

Overlapping downregulated genes: 15

RR9 comparisons: space flight vs ground

Aging dataset comparisons: 12M,18M,24M vs 3M