1 of 11

Marc Vaudel

Center for Medical Genetics and Molecular Medicine, �Haukeland University Hospital, Bergen, Norway

�Center for Diabetes Research, Department of Clinical Science, University of Bergen, Norway

Gene RNA protein abundance

22.06.21

THREE LEVELS [2015]�TYLER HOBBS

2 of 11

Comparing gene, RNA, and �protein abundances

3 of 11

Gene > transcript > peptide > protein > proteoform

What is the right level to compare transcript and protein abundance?

3

4 of 11

Genotype vs. peptide abundance

What can explain the difference between expected and observed distributions?

4

5 of 11

Copy number attenuation

What can be the source of copy number attenuation?

5

6 of 11

RNA vs. protein abundance

How can we take advantage of the RNA-protein correlation – or lack thereof?

6

7 of 11

RNA vs. protein abundance

7

8 of 11

From genes to proteoforms

8

9 of 11

From genes to proteoforms

9

How can we take advantage of the combination of these analytical layers?

10 of 11

From genotypes to phenotypes

10

How would you use the association of genetic variants with biomarkers?

11 of 11

Acknowledgement