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Libraries in pooled cultivations for salt tolerance

  • Targeting most genes in E. coli (4,191 total)
  • 5 x promoter ladder (varying strength)
  • 2 x KO (positions 5 and 15 of each gene)
  • 25K total designs

Promoter

Strength

J23112

1X

J23116

396X

J23106

1,185X

J23101

1,791X

J23100

2,547X

CONFIDENTIAL & PROPRIETARY

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25K biological responses in a single experiment

Biological Response

Slope/SE

Increasing Promoter Strength 🡪

Knock-Outs

4K genes

Enriched

Depleted

waaZ

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Biological response profiles by gene clusters

Source here

Uniform Depletion

Resistance Susceptibility

Uniform Enrichment

Resistance Conferring

UMAP Clustering (2,800 Genes x 7 Edit Types)

CONFIDENTIAL & PROPRIETARY

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