Chromatography

Lesson 1: Chromatography

Before

After

Chromatography

Objectives�

1. Explain how chromatography works
2. Calculate the Rf values

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What do you already know about chromatography?

My Word Cloud - AhaSlides

Sticky �notes

Creativity

Recap

Experiment

Try paper chromatography for yourself 🧪.

amrita.olabs.edu.in/?sub=79&brch=17&sim=124&cnt=4

First see the next page

Practice first

Hands-on activity

Before we go - recap

• Why are plants green?

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Experiment

1. Scan the code
2. Run the simulation
3. Tick the boxes, identify the 4 bands
4. Answer the quiz question
5. Rerun the experiment a second time

What mark do you want to get on your GCSE?

GCSE

Do you like perfumes?

Group Work

Watch the video, and then let’s discuss it

https://www.youtube.com/shorts/jWuRMqIbyhI

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Discuss in groups of four

• What’s your favorite perfume?
• How to tell if the perfume is cheap or expensive?
• What did you find out from the video?
• How to use chemistry to avoid overpaying 10 times for the raw materials?
• What did the company do with the fraudulent supplier?

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Collaboration

Critical thinking

Communication

Creativity

Calculating the Rf

• Rf stands for the retention factor

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Examples

Calculate the Rf

• The Rf is measured from where the starting point to the center of the spot
• I do: chlorophyll A

Rf = 27/62 = 0.43

2. We do: xanthophyll �Rf = 56/62 = 0.90

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3. You do: chlorophyll B

0.30��

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Key Words

• Chromatography
• Rf
• Retention factor
• Solvent
• Band
• Chlorophyll
• Xanthophyll

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Summary

Today we��🔲 Separated chlorophyll A from chlorophyll B

🔲 Solved a GCSE test question

🔲 Discussed the use of chromatography in perfumery

🔲 Calculated the Rf

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Who will win a star? ⭐⭐⭐⭐⭐

Join the quiz��Library - Kahoot!

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