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Introduction to the Light Microscope

Care
Parts
Focusing
Magnification

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The light microscope, uses visible light and a system of lenses to magnify images of small samples.

Light microscopes are the oldest design of microscope and were invented in the 17th century.

Can be monocular or binocular.

Light Source

Lenses

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Used to view large specimens. Usually only magnifies up to 4x.

Stereoscope or Dissecting Scope

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Always carry with 2 hands, one hand on the arm, one on the base.
Only use lens paper for cleaning
Do not force knobs
Always store covered
Keep objects clear of desk and cords

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Magnification

The total magnification is calculated by multiplying the ocular x the objective.

Scanning → 10 x 4 = 40x

Low Power → 10 x 10 = 100

High Power →

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Place the slide on the stage (stage clips are not necessary)
Click nosepiece to the lowest (shortest) setting – Scanning Objective
Look into the Eyepiece
Use the Coarse Focus to bring object into view.

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5. (Once the slide is focused) Rotate the nosepiece to the low power objective (medium sized)

6. Refocus using the coarse (large) knob

7. Move slide to get a centered view

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**All the steps above must be completed before you attempt to focus on high power**

8. Click the high power objective into place, it should be very close to the stage. �9. Only use the fine adjustment knob (small) to bring object into focus.

What can you find on your slide?

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Eyepiece

Arm

Stage

Coarse Focus

Fine Focus

Base

Body Tube

Revolving Nosepiece

Low Power

Scanning

High Power

Stage Clips

Diaphragm

Light

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