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A 3D BIO-PRINTED SPHEROIDS BASED PERFUSION IN VITRO LIVER ON CHIP FOR DRUG SCREENING

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Introduction

Method

Result

Conclusion

Outline

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Introduction

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The function of human Liver

PRODUCTION

Detoxification

Transformation

Excretion

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Introduction

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1.Difficulty to do drug testing

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2.Difficulty to study the signal path

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3. Drug influence can’t be detect

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2D Liver Disadvantage

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2

Transwell chambers

Introduction

The liver is the main site of drug metabolism, and the

key functional unit of liver is lobule

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Method

HepG2(3D)

HUVEC (2D)

pressure sensitive

adhesive tape

PET

membrane

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2D&3D Cell Culture

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Purose:

USED (HepG2)&(HUVEC).

1. 2D cultures of HUVECs

were performed on the bottom side of the membrane .

2. 3D spheroid cultures were performed by the 3D bio-printer on the top side of the transwells.

100 spraying times

4 spray heads work

Print height:5mm

Diameter:400um

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Check the cell Viability

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  • LIVE-calcein-AM (green)

calcium esterase

  • Dead-ethidium homodimer-1 (red)

could not pass through membrane

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Mimicking the microenvironment in a chip

HUVEC cells mainly perform the function as a barrier to

allow substances to through.

To mimic the environment we perfused the fluid to rebuild it.

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Compare 2D & 3D Liver

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Conclusion

  • perfusion liver on chip device which could be appropriated for the 3D bio-printer for drug screening
  • Two types of cells, HepG2 and HUVEC, The results shown that the viability of the two cells in this device above 85%
  • The characterized marker ALB and UREA IS better than 2D

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So Protect Your Liver

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Thank you for your listening!��Q&A�