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SteamWatch Water Quality Monitoring Internship

Lily Raphael, Riley Samples, and Fiona Wolter

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About the Watershed Institute

The goal of The Watershed Institute is keeping water clean, safe and healthy is the heart of our mission. We work to protect and restore our water and natural environment in central New Jersey through conservation, advocacy, science and education.​

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What is a watershed?

A watershed is an area of land where all the water drains into a specific body such as a river, lake, or ocean. It describes the entire land area that contributes water to a particular body of water.

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StreamWatch

Biological Team (BAT)

Chemical Team (CAT)

Bacterial Team (BACT)

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Monitors macroinvertebrate populations and uses pollution tolerance values to quantify water quality

Measures water chemistry

    • Temperature, Nitrate, Phosphate, pH, Turbidity, Dissolved Oxygen,Conductivity

Measure E. coli levels, indicator for fecal contamination

Monitors harmful algal blooms (HABs)

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Lab work

  • Nutrients: Chloride, Phosphorus, Nitrate (Total Nitrogen - Nitrite)
  • E.coli Counts
  • Turbidity

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Field work

Fluorometer meter

  • Measures: phycocyanin levels, which is the pigment produced by HAB-causing cyanobacteria

Hanna Meter (Multiparameter)

  • Measures: temperature, pH, dissolved oxygen, conductivity, and total dissolved solids

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Wargo Pond Monitoring

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Gather biweekly data using the fieldwork meters in conjunction with taking samples for E.coli, turbidity, and nutrient testing

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Purpose

  • Monitor for harmful algal blooms (HABs)
  • Determine the effect of water flow in and out of the pond

Map of sample locations around Wargo Pond

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The Gallery Discrete Analyzer

Photometry: using light absorbance to detect analyte concentration

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Process:

  1. Samples are loaded into wells on racks
  2. The machine mixes samples with reagents, producing a color change
  3. The mixtures are transferred into cuvettes
  4. Light passes through the cuvettes, and absorbance is measured at specific wavelengths
  5. Blanks and a calibration curve are applied to convert absorbance into nutrient concentrations

Fiona Wolter

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Challenges with this Machine

Unclear what to do with a high blank result

Interpreting test results

Frequent Variance in Test Results

Inconsistencies in the same test that is rerun multiple times

Sample Data

Run blank samples in each rack but no standard for what makes a high blank

Blanks

Unclear what distinguishes a low result from a blank

High and low test inconsistencies

Unclear protocol for deciding when to accept vs rerun tests

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Addressing these Challenges

Calculate limit of detection (LoD) and limit of quantification (LoQ) using blank sample data and the corresponding calibration curves

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Create decision support sheets for accepting vs rerunning test results

Investigate the potential for instrumental drift by charting calibration data from the past five years

Test different preparation methods to evaluate sample non-homogeneity and determine how to minimize this source of error

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Calibration Analysis Results

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Sample Preparation Data Results

*Run 4 for shake was 356.3261

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Making StreamWatch Data Accessible

A goal of The Watershed this year is to make all of their data publicly available. The Watershed has around 40 year of collected data, but none of it is publicly available. The audience for this data consisted of both professional lab group as well as younger educational groups.

To tackle this goal, the first thing I did was create a guide for understanding the data, so that any audience has the opportunity to analyze the data accurately.

This guide covers every column in the master data spreadsheet, and is accessible in both pdf form and directly on the excel sheet.

I sorted the data in to 27 different subsets, as well one sheet that contains all data.

Riley Samples

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Making StreamWatch Data Accessible

To further increase the ease of use of The Watershed’s data, I developed a similar guide for data visualization. This guide, provides detailed information on how to clean, subsect, visualize, and analyze the data directly in excel.

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Inside the modeling instructions, I created some of my own visualizations to provide inspiration and an expression of the excel modeling capabilities

These instructions, similar to the data dictionary, are available both directly inside the All Streamwatch Data spreadsheet, and also in PDF format.

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Making StreamWatch Data Accessible

The last and largest part of making this data accessible was creating a public portal that allows the data to be downloaded. To do this, I utilized a website made by a former intern, originally intended for informing Streamwatch volunteers. This was done through ArcGIS. Here, I developed an additional webpage where the data is publicly accessible, both in browser and downloadable formats.

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Also accessible through this dashboard is a document continuing the history of The Watershed’s data collection techniques, which enables users to search for possible explanations to unexpected data shifts.

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Benthic Freshwater Macroinvertebrates

Bottom

dwelling

Large enough to be seen with the naked eye

No backbone

Pollution Tolerance of

Macroinvertebrates

Macroinvertebrates and Water Quality

Pollution Sensitive

0-4

Pollution Tolerant

6-10

Pollution “Neutral”

4-6

Crayfish

(5)

Scud

(7)

Stonefly nymph

(1)

Found in lakes, steams, rivers, etc.

  • Long term stationary organisms
  • Easy to collect

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Macroinvertebrate Indices

New Jersey Impairment Score (NJIS)

Coastal Plains Macroinvertebrate Index (CPMI)

High Gradient Macroinvertebrate Index (HGMI) - Genus

High Gradient Macroinvertebrate Index (HGMI) - Family

Map of the index boundaries throughout New Jersey

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Indices Calculations

HGMI (genus)

HGMI (family)

CPMI

No further calculations

Raw Data

Select a sample of 100 organisms

Calculate NJIS

Contains Genus Information

Only Contains Family Information

High Gradient Site

Contains Genus Information

Only Contains Family Information

Low Gradient Site

High Gradient

Only Family

Information

Sample Calculation

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Learning Takeaways

Water Quality monitoring involves a wide variety of steps and metrics! We were able to see this process through from sample collection and field work to lab work to data analysis, allowing us to better understand the different components of this kind of work.

Our personal projects gave us additional opportunities to explore instrumentation, design experiments, clean and visualize data, and work with software tools such as Excel and ArcGIS.

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Acknowledgements and Questions

  • Jian Smith, StreamWatch Program Coordinator
  • Erin Stretz, Assistant Director of Science
  • Steve Tuorto, Director of Science and Stewardship
  • Michael Kim, Science Fellow
  • Emily Ahmetaj, HMEI Internship Program Coordinator