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Determine the Germicidal Limits of Plant Leaf Extracts for Drinking Water Sterilization

Presented by-

  • Yogeeta Gupta (10BB22A02034)
  • Mukta Yadav (10BB22A02016)
  • Nikita Bhor (10BB22A02020)
  • Arushi Goel (10BB22A02009)

CSIR 800

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  • Investigate the efficacy of unconventional germicidal plant leaf extracts in eliminating harmful bacteria commonly found in contaminated drinking water.
  • Determine the minimum concentration of each extract required to achieve a significant reduction in bacterial viability (germicidal limit).

OBJECTIVES

FIG

Ficus carica

KARELA

Momordica charantia

GUAVA

Psidium guajava

INDIAN BAEL Aegle marmelos

POMEGRANATE

Punica granatum

INTRODUCTION

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Preparation of Plant Extract

Performing MIC assay on selected test bacteria

Antibacterial Assay

Cytotoxicity assay

  • Prepare leaf extracts (by boiling in water)
  • Standardizing the preparation protocol to minimize the degaradation of active compounds.
  • Select commonly found bacteria in contaminated water (E. coli, S. aureus, and S. typhi)
  • Study the Minimal Inhibitory Concentration of extracts on these bacteria
  • Treat the water samples (collected from different sources) with different conc. of extracts for varying time periods (15 min, 30 min, 1 h)
  • Enumerate viable bacteria using CFU assay
  • Evaluate the potential cytotoxicity of extracts (toxicity to human cells)

METHODOLOGY

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