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Antenatal bleeding & Isoimmunization

31-year-old, G1P0, 14+3 weeks, presents with vaginal bleeding.

Vitals: 73 bpm, BP 118/76, RR 16, temp 36.5℃

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DDx for Antepartum bleeding*

  • Ectopic pregnancy
  • Spontaneous abortion
  • Implantation bleed
  • Gestational trophoblastic neoplasm
  • Subchorionic hematoma
  • Non-obstetric causes (infection, trauma from intercourse, cervical cancer, rectal bleeding, etc)

  • Placental abruption
  • Uterine rupture
  • Placenta previa
  • Vasa previa
  • Stillbirth
  • Bloody show
  • Non-obstetric causes (infection, trauma from intercourse, cervical cancer, rectal bleeding, etc)

<20 weeks

>20 weeks

*this list is not exhaustive, and lists only the most common causes of antepartum bleeding.

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What do you want to know on history?

  • Onset and duration of bleeding?
  • Intermittent or continuous?
  • Amount of blood loss (spotting? soaking through pads?)
  • Colour of blood (bright or dark red?)
  • Passage of clots or tissue?
  • Provoking factors (recent intercourse, injury, increased activity…)?
  • If fetal movement has started, has there been a decline in movement?
  • ROS: abdominal pain, fever/chills, fatigue, syncope

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What do you want to know on history?

  • ABCDs! Activity, (Bleeding), Contractions, Discharge!
  • Medical history
  • Surgical history
  • Obstetrical history
    • EDD for current pregnancy?
    • Natural or artificial conception (e.g., IVF)?
    • History of ectopic pregnancy?
    • Previous C-sections?
  • Gynecological history
    • Previous STIs or PID?
    • Previous gynecological procedures?
    • Diagnosis of a gynecological condition (e.g. fibroids, polyps, …)
  • Medications
  • Allergies
  • Family history
  • Social history

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Make sure to remember…

…we should be asking questions to screen for life-threatening causes like ectopic pregnancy!

  • Previous ectopic pregnancy
  • Previous pelvic infections, STIs, endometriosis
  • Previous pelvic/tubal surgery
  • History of infertility
  • IVF
  • Smoking
  • Age >35
  • IUD in situ

Recall the risk factors of an ectopic pregnancy and let this guide your history

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History findings

The patient has had continuous spotting for the past 2 days. The blood is bright red, she has not noticed any clots or tissue being passed, and the last time she had intercourse was over a week ago. She is not having any abdominal pain or abnormal discharge other than the bleeding. All other symptoms on ROS were negative.

This is her first pregnancy, conceived by IVF. EDD is March 27th. She is very concerned about a potential miscarriage. She has a history of chronic migraines that she manages with sumatriptan. She has had a tonsillectomy as a child, and no other surgeries. She has no allergies. Gynecological and family history are unremarkable.

She lives in Edmonton with her partner. She use to smoke ½ pack a day since she was 18, but she quit 1 year ago when she was struggling with fertility. She used to have 1-2 drinks per week but has not had any since she found out she was pregnant. She does not use any other substances.

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What to look for on physical exam?

    • Bruising (Cullen/Grey Turner’s sign)
    • Distension
    • Peritonitis

Abdomen

    • External examination for signs of trauma
    • Bimanual exam - cervical motion tenderness, adnexal mass/tenderness
    • Speculum exam - look for the source of bleeding, signs of trauma, purulent/foul discharge, open internal cervical os

Pelvis

    • Vitals – fever, tachycardia, tachypnea, hypotensive

General

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Physical exam findings

    • No scars, bruises, or other skin changes.
    • Abdomen is soft, not distended, mildly tender in the lower quadrants on palpation.

Abdomen

    • Dried blood at the introitus
    • No cervical motion or adnexal tenderness.
    • No adnexal masses
    • Few millimeters of blood present in the vaginal vault.
    • Internal cervical os is closed, no active bleeding.
    • No signs of trauma.

Pelvis

    • Vitals are stable
    • She appears anxious but otherwise well

General

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What investigations do you want to order?

Labs: CBC-diff, serum hCG, type + screen

Imaging: transabdominal US

US report

Estimated GA: 14 weeks 3 days

EDD: 03-26-2024

Placenta: posterior

Hyperechoic subchorionic hematoma present, approximately 17% of the size of the gestational sac.

CBC

WBC 7.3

RBC 3.9

HGB 102 (L)

HCT 0.40

MCV 84

RDW 14.1

PLT 216

serum hCG

28,774 IU/L

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Investigations

US report

Estimated GA: 14 weeks 3 days

EDD: 03-26-2024

Placenta: posterior

Hyperechoic subchorionic hematoma present, approximately 17% of the size of the gestational sac.

You remember from pre-clerkship that a subchorionic hematoma is when blood accumulates between the uterine wall and the chorion membrane that encloses the amniotic sac.

You also remember that this is a common cause of bleeding in early pregnancy and often resolves spontaneously!

Since the patient is stable and not symptomatic, you propose that the patient be discharged.

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But wait…

Your senior resident reminds you to check the patient’s Type and Screen!

You pull up the report on Netcare and read the following:

ABO Group:

O

Rh:

Negative

How does this change your management?

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Isoimmunization

Recall that Rh incompatibility between mom and baby can result in Rh isoimmunization!

  • An Rh- mother may develop antibodies if Rh+ blood from the fetus is passed into their circulation

Causes

  • Labor/delivery
  • Ruptured ectopic pregnancy
  • Placental abruption
  • Abortion (spontaneous or elective)
  • Procedures (CVS, amniocentesis)
  • **anything leading to fetomaternal hemorrhage!
  • When the mother is exposed for a second time (i.e. in a subsequent pregnancy), these anti-Rh antibodies can cross the placenta and trigger destruction of RBCs in the fetus

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Isoimmunization

Clinical outcomes range from mild anemia to hydrops fetalis, including:

  • Pallor (due to anemia)
  • Jaundice
  • Hepatosplenomegaly
  • Respiratory distress
  • Coagulopathies
  • Hypoglycemia
  • Kernicterus
  • Therefore, all mothers should have a Type + Screen at their first prenatal visit.
  • SOGC guidelines indicate routine administration of Rhogam at:
    • 28 weeks gestation
    • Within 72 hours of delivery

This can lead to Hemolytic Disease of the Fetus & Newborn

Note: Hemolytic anemia can also occur with incompatibility between ABO and other antigens (anti-kell, duffy, kidd…). See here for more info.

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Management

Management of Antepartum Bleeding

  • Assess ABCs and vitals - is the patient stable?
  • Check Type & Screen - is the patient Rh-?
  • If the patient is stable and Rh-
    • Run a Kleihauer-Betke test (after 20w) and administer Rhogam
    • Discharge with instructions to return if bleeding worsens or new symptoms arise
    • Arrange for f/u ultrasounds to monitor the size of the subchorionic hematoma

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Management

Run a Kleihauer-Betke test (after 20w) and administer Rhogam

  • A Kleihauer-Betke test will screen maternal blood for the presence of fetal RBCs and determine the severity of a fetomaternal hemorrhage
  • You can use the results to calculate the dose of Rhogam that should be administered:

*round to the nearest whole number and add 1

# of vials* = (% fetal cells present) x (maternal blood volume)

30 mL per vial

For example, if KB test shows 0.9% fetal cells

Maternal blood volume is ~4500 mL

# vials = (0.9% x 4500 mL) / 30 mL = 1.35 (round to 1 and add 1)

Administer 2 vials of Rhogam

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Management

Management of Antepartum Bleeding

  • Assess ABCs and vitals - is the patient stable?
  • Check Type & Screen - is the patient Rh-?
  • If the patient is stable and Rh-
    • Administer Rhogam
    • Discharge with instructions to return if bleeding worsens or new symptoms arise
    • Arrange for f/u ultrasounds to monitor the size of the subchorionic hematoma

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Practice

CASE 2: A 30-year old G1P0 patient presents to the ER with sudden onset moderate back pain and bright red vaginal bleeding that began 1 hour ago.

Pertinent History:

Patient has had no prenatal care, but estimates their LMP was around 8 months ago. Endorses methamphetamine and cannabis use in pregnancy. Previous history of GERD, depression and psoriasis. Had an appendectomy at 14 years old. Takes sertraline 100 mg and prenatal vitamins daily.

Physical Exam:

BP 100/72 mmHg, HR 105 bpm, RR 18/min, temp 36.5℃, SpO2 97%

GCS 15, pale and mildly diaphoretic

SFH is 36 cm

Abdomen: No palpable uterine contractions, tenderness along the lower abdomen.

Vaginal: Bright red blood present in vaginal vault, active bleeding from the

cervical os. Cervix is 1cm dilated, 30% effaced, station -4

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Practice

CASE 2: A 30-year old G1P0 patient presents to the ER with sudden onset moderate back pain and bright red vaginal bleeding that began 1 hour ago.

Investigations:

Labs: Hb 108, INR 1.2

Type & Screen: AB, Rh-

NST: FHR 142 bpm, moderate variability, no accelerations or decelerations present

Transabdominal US: Cephalic presentation, retroplacental hemorrhage

How do you manage this patient?

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Practice

CASE 2: A 30-year old G1P0 patient presents to the ER with sudden onset moderate back pain and bright red vaginal bleeding that began 1 hour ago.

How do you manage this patient?

US confirmed placental abruption. Patient is mildly hypotensive and tachycardic, pale and still actively bleeding. No signs of imminent delivery.

  • Prep for C-section!
    • IV pen G not required for GBS prophylaxis
  • Prepare for high risk of PPH and potential transfusion protocol
  • Run KB test and administer Rhogam within 72h of delivery

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Practice

CASE 2: A 30-year old G1P0 patient presents to the ER with sudden onset moderate back pain and bright red vaginal bleeding that began 1 hour ago.

C-section was performed.

Mom required transfusion of 2 units packed RBCs.

Baby required resuscitation and was transferred to NICU.

A Kleihauer-Betke test is ordered 24h after the procedure and shows 4.6% fetal cells present in maternal blood. How much Rhogam should be administered? (note: patient’s height is 155 cm and weight is 61 kg)

Estimated maternal blood volume: 3.5L

# vials = (4.6% x 3500 mL) / 30 mL = 5.36 → administer 6 vials Rhogam

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Resources

Interested in learning more? Check out these articles!

SOGC Prevention of Rh Alloimmunization Guidelines: https://www.jogc.com/article/S1701-2163(17)31111-8/abstract

�Placental Abruption - StatPearls

https://www.ncbi.nlm.nih.gov/books/NBK482335/

Battula SP, Mohammed N, & Datta S (2021). Antepartum haemorrhage. Obstetrics, Gynaecology & Reproductive Medicine, 31(4), 117-123.

https://www.sciencedirect.com/science/article/pii/S1751721421000348?casa_token=K3IiW867YjAAAAAA:NfFXrpD6bUwvzmPEneYGpHnGu54J_aNg7-jnCavc_MQ5jG-CBqWu1_Q-6b7_SREXL4YCfec#sec1

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Authors

Claudia Turco

Rahim Janmohamed