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THINK ABOUT IT

What is an inheritance?

It is something we each receive from our parents—a contribution that determines our blood type, the color of our hair, and so much more.

Where does an organism get its unique characteristics?

An individual’s characteristics are determined by “factors” that are passed from one parental generation to the next.

The delivery of characteristics from parent to offspring is called heredity.

The scientific study of heredity, known as genetics, is the key to understanding what makes each organism unique.

A trait is a variation of a particular characteristic of an individual, such as AB blood, red hair and purple flowers.

Lesson Overview

The Work of Gregor Mendel

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Gregor Mendel

  • Father of genetics (Austrian monk)
  • Applied experimental approach to the question of inheritance.
    • Bred pea plants and recorded inheritance patterns in the offspring – for 7 years! (“model system”)

Lesson Overview

The Work of Gregor Mendel

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Mendel Worked with True-Breeding Pea Plants

  • When self-fertilized, a true-breeding plant produces offspring identical in appearance to itself generation after generation

Lesson Overview

The Work of Gregor Mendel

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Mendel Cross-Fertilized Plants by Hand

Cross-pollination allowed Mendel to breed plants with traits different from those of their parents and then study the results.

Lesson Overview

The Work of Gregor Mendel

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Monohybrid Cross: Parents Differ in Only One Character

  • Hybrids: Offspring of 2 different true-breeding varieties

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The Work of Gregor Mendel

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Mendel Examined Inheritance Patterns of 7 Pea-Plant Characters

  • For each character, 1 of the 2 parent traits disappeared in the F1 hybrids, but . . .

Lesson Overview

The Work of Gregor Mendel

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. . . reappeared in ~1/4 of the F2 generation ……why?

Mendel’s 2nd experiment told the story mathematically!

Lesson Overview

The Work of Gregor Mendel

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Mendel’s principles (Laws)

1. Alleles are alternative forms of “factors” (genes) which are passed from parent to offspring.

    • Ex: Purple (P) or white (p) flower color

An organism has a pair of alleles that controls each character trait.

    • One allele comes from each parent
    • Homozygous: 2 alleles for that character are the same (PP or pp)
    • Heterozygous 2 alleles are different (Pp)

Lesson Overview

The Work of Gregor Mendel

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Alleles are Alternative Versions of a Gene

Lesson Overview

The Work of Gregor Mendel

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Mendel’s principles (Laws)

1. Alleles are alternative forms of “factors” (genes) which are passed from parent to offspring.

    • Ex: Purple (P) or white (p) flower color

An organism has a pair of alleles that controls each character trait.

    • One allele comes from each parent
    • Homozygous: 2 alleles for that character are the same (PP or pp)
    • Heterozygous 2 alleles are different (Pp)

2. Dominant allele masks the recessive allele in a heterozygous individual

    • P = dominant allele
    • p = recessive allele

Lesson Overview

The Work of Gregor Mendel

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Dominant and Recessive Traits

In Mendel’s experiments, the allele for tall plants was dominant and the allele for short plants was recessive.

Lesson Overview

The Work of Gregor Mendel

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Dominant and Recessive Traits

In Mendel’s experiments, the allele for tall plants was dominant and the allele for short plants was recessive. Likewise, the allele for yellow seeds was dominant over the recessive allele for green seeds

Lesson Overview

The Work of Gregor Mendel

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Mendel’s principles (Laws)

1. Alleles are alternative forms of “factors” (genes) which are passed from parent to offspring.

    • Ex: Purple (P) or white (p) flower color

An organism has a pair of alleles that controls each character trait.

    • One allele comes from each parent
    • Homozygous: 2 alleles for that character are the same (PP or pp)
    • Heterozygous 2 alleles are different (Pp)

2. Dominant allele masks the recessive allele in a heterozygous individual

    • P = dominant allele
    • p = recessive allele

3. Principle of segregation: The 2 alleles for a character segregate (separate) during the formation of gametes (sex cells).

    • Each gamete carries only 1 allele for each character.
    • The union of gametes during fertilization reforms allele pairs in the offspring.

Lesson Overview

The Work of Gregor Mendel

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Segregation = Meiosis

How are different forms of a gene distributed to offspring?

During gamete (sex cell) formation, the alleles for each gene segregate from each other, so that each gamete carries only one allele for each gene.

Mendel assumed that a dominant allele had masked the corresponding recessive allele in the F1 generation.

The reappearance of the recessive trait in the F2 generation indicated that, at some point, the allele for shortness had separated from the allele for tallness.

Lesson Overview

The Work of Gregor Mendel

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Let’s assume that each F1 plant—all of which were tall—inherited an allele for tallness from its tall parent and an allele for shortness from its short parent.

The Formation of Gametes (Meiosis)

When each parent, or F1 adult, produces gametes (meiosis), the alleles for each gene segregate from one another, so that each gamete carries only one allele for each gene.

One gamete from each parent will randomly combine during fertilization.

The F2 generation has new combinations of alleles.

Lesson Overview

The Work of Gregor Mendel

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Probability and Punnett Squares

How can we use probability to predict traits?

Probability is the likelihood that a particular event will occur.

Punnett squares use mathematical probability to help predict the genotype and phenotype combinations in genetic crosses.

The probability of a particular combination is the product of the separate probabilities for each coin.

Ex: Probability of 2 heads showing is 1/2 x 1/2 = 1/4

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The Work of Gregor Mendel

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Punnett Square: Step 1) show the parent genotypes & cross [Pp X Pp]

Step 2) draw square & separate parent alleles into possible gametes [ P or p]

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The Work of Gregor Mendel

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Step 3) show all possible combinations of gametes� (fertilization events)

Step 4) record Genotype and Phenotype ratios

(pairs of alleles/genes) G: ¼ PP, ½ Pp, ¼ pp

(physical appearance) P: ¾ Purple, ¼ White

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The Work of Gregor Mendel

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1) Write the genotypes of the two organisms that will serve as parents in a cross.

In this example we will cross a male and female osprey that are heterozygous for large beaks. They each have genotypes of Bb.

Bb and Bb

2) Draw a table and determine what alleles would be found in all of the possible gametes that each parent could produce.

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The Work of Gregor Mendel

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3) Fill in the table by combining the gametes’ genotypes.

4) Determine the genotypes and phenotypes of each offspring.

Calculate the percentage of each. In this example:

G: ¼ BB, ½ Bb, ¼ bb

P: ¾ large beaks, ¼ small beaks

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The Work of Gregor Mendel

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4) Mendel’s principle of Independent Assortment

How do alleles segregate when more than one gene is involved?

The principle of independent assortment states that genes for different traits can segregate independently during the formation of gametes.

Mendel performed an experiment that followed two different genes as they passed from one generation to the next.

Because it involves two different genes, Mendel’s experiment is known as a two-factor, or dihybrid cross. Single-gene crosses are monohybrid crosses.

Lesson Overview

The Work of Gregor Mendel

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The Two-Factor Cross: F1

Mendel crossed true-breeding plants that produced only round yellow peas with plants that produced wrinkled green peas.

The round yellow peas had the genotype RRYY, which is homozygous dominant.

The wrinkled green peas had the genotype rryy, which is homozygous recessive.

Step 1) RRYY X rryy

Step 2) FOIL method: RRYY = RY, RY, RY, RY

rryy = ry, ry, ry, ry

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The Work of Gregor Mendel

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The Two-Factor Cross: F1

Step 4:

100% of the F1 offspring had the phenotype round yellow peas. These results showed that the alleles for yellow and round peas are dominant over the alleles for green and wrinkled peas.

The Punnett square shows that the genotype of each F1 offspring was 100% RrYy, heterozygous for both seed shape and seed color.

Step 3) combine all possible gametes

- keep like letters together

- capital first

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The Work of Gregor Mendel

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The Two-Factor Cross: F2

Mendel then crossed the F1 plants to produce F2 offspring.

Step 1) RrYy X RrYy

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The Work of Gregor Mendel

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Principle of Independent Assortment

  • Since the alleles for different genes are sorted into the gametes independently of one another; during gamete formation in an F2 cross, a particular allele for one character can be paired with either allele of another character.

    • Step 2) FOIL method:

RrYy = RY, Ry, rY, ry

RrYy = RY

Ry

rY

ry

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The Work of Gregor Mendel

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Step 3) combine all possible gametes

- keep like letters together

- capital first

Step 4) Four Possible Phenotypes

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The Work of Gregor Mendel

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A Summary of Mendel’s Principles

What did Mendel contribute to our understanding of genetics?

Mendel’s principles of heredity, observed through patterns of inheritance, form the basis of modern genetics.

1) The inheritance of biological characteristics is determined by individual units called genes, which are passed from parents to offspring.

2) Where two or more forms (alleles) of the gene for a single trait exist, some forms of the gene may be dominant and others may be recessive.

3) In most sexually reproducing organisms, each adult has two copies of each gene—one from each parent. These genes segregate from each other when gametes are formed.

4) Alleles for different genes usually segregate independently of each other.

Lesson Overview

The Work of Gregor Mendel

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A Summary of Mendel’s Principles

At the beginning of the 1900s, American geneticist Thomas Hunt Morgan decided to use the common fruit fly as a model organism in his genetics experiments.

 

The fruit fly was an ideal organism for genetics because it could produce plenty of offspring, and it did so quickly in the laboratory.

Before long, Morgan and other biologists had tested every one of Mendel’s principles and learned that they applied not just to pea plants but to other organisms as well.

 

The basic principles of Mendelian genetics can be used to study the inheritance of human traits and to calculate the probability of certain traits appearing in the next generation.

Lesson Overview

The Work of Gregor Mendel