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MASTER'S COURSE OVERVIEW • MODULES 1 & 2

Physical & Chemical Methods: Optics & Metabolomics

A comprehensive guide to super-resolution microscopy, optical-spectral analysis, HPLC separation, mass spectrometry, and systems biology metabolomics.

Structure

2 Educational Modules

Format

22h Lectures • 22h Lab Work

Target Level

Second (Master) Degree

AI - assisted content*

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MODULE 1 • TOPIC 1

Advanced Visualization & Micro-Object Imaging

SIM, STED, SMLM: Breaking diffraction barriers for nanometer resolution imaging.

Sub-cellular detail: Transmission sectioning and scanning surface topographies.

Vitrified structure: Native biomolecular mapping without dye staining artifacts.

Super-Resolution Microscopy

Electron Microscopy (TEM / SEM)

Cryo-Electron Microscopy (Cryo-EM)

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MODULE 1 • TOPIC 2

Optical-Spectral Analysis & Biophysical Cytometry

Spectrophotometry

Bouguer-Lambert-Beer: Protein and DNA concentration quantification.

Raman Scattering

Label-free fingerprint: Chemical vibrational signature analysis.

Flow Cytometry

Single-cell profiling: Laser sorting and fluorescence population analysis.

Fluorometry

Emission kinetics: High-sensitivity detection of exometabolites.

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MODULE 2 • TOPIC 3

Chromatography & Mass Spectrometry (MS)

HPLC & Gas Chromatography

Fine-sorbent separation: Purifying bacteriocins, organic acids, and cellular sugars.

Ionization Modes (MALDI & ESI)

Laser & electrospray: Soft ionization for intact biological macromolecules.

Mass Analyzers (Orbitrap & QMF)

Quadrupole & FT-MS: Exact m/z determination and adduct identification.

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MODULE 2 • TOPICS 4 & 5

Metabolomics in Systems Biology & Medicine

NMR Spectroscopy

Non-destructive analysis: Preserves metabolite structure for magnetic resonance profiling.

Targeted vs Untargeted

Comprehensive phenotyping: Metabolic fingerprinting and key pathway profiling.

Therapeutic Targets

Biomarker discovery: Disease diagnosis and bio-antimicrobial compound mapping.

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EVALUATION & COMPETENCIES

Modules 1 & 2 Practical Mastery

40 pts

Test 1: Module 1

Optical & spectral analysis theory.

40 pts

Test 2: Module 2

Chromatography & mass spectrum decoding.

20 pts

Lab Protocols

GFP purification & metabolomic data analysis.