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Carolynn (Carrie) Harris

PhD Candidate Dartmouth College

Tardigrades &

ASTROBIOLOGY

How’s everyone feeling?

Annotate the alien that gets it

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ASTROBIOLOGY

the origin, evolution, distribution,

& future of life in the universe

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Are we alone?

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How many stars in the sky?

How many planets are in the

“habitable zone”?

How many stars have planets?

2 billion trillion

2 x 1023

40% of stars have at least 1 planet, many have more

About 3 x 1023 planets

How many planets have life?

Just Earth!

… as far as we know

60 billion

6 x 1010

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INGREDIENTS FOR LIFE

chemical

building blocks

CHNOPS elements

carbon, hydrogen, nitrogen,

oxygen, phosphorus, & sulfur

energy

Organic matter (food!), sunlight, chemical energy

time

What makes a planet “habitable”?

solvent

Water (H2O)

But also maybe ammonia (NH3), methanol (CH3OH),

or sulfuric acid (H2SO4)…

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CONDITIONS FOR LIFE

temperature

What makes a planet “habitable”?

humidity (water)

pH (acid or base)

salinity

pressure

radiation (sun)

Type your ideas in the chat

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Organisms that thrive in “extreme” conditions

What are the limits of life?

EXTREMOPHILES

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Extremophilic micro-eukaryote

TARDIGRADES

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TARDIGRADES

Survival Claims to Fame

Extreme hot and cold

High and low pressure

The vacuum of space

Radiation

Lack of water - dehydration

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TARDIGRADES

Biology & Ecology

Eukaryote: Micro-animal (< 1mm)

Evolved 600 million years ago

Live everywhere, prefer lichens & mosses

Omnivores – eat plants, bacteria, smaller tardigrades

Anatomy – head, 8 legs, 3 body segments, 1 caudal segment

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TARDIGRADES

Dormant “tun” state aka cryptobiosis

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TARDIGRADES

Tardigrade from Lake Fryxell –

A permanently ice-covered lake in Antarctica

Video Credit:

Dr. Wei Li (MCM LTER)

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AI Image

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In 2019, Israel's Beresheet lunar lander 

crash landed on the moon.

It’s payload included tardigrades in cryptobiosis.

Scale model of Beresheet

Landing site – pre & post impact

Credit: Wikipedia

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Did the tardigrades survive the crash?

Are they still alive today?

(Vote in the zoom poll)

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What type of conditions are the

tardigrades be exposed to?

Did the tardigrades survive the crash?

Are they still alive today?

(Type your ideas in the chat)

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Let’s examine data from real experiments that tested the “extreme” limits of tardigrades

Each small group will receive raw data from a

real experiment that exposed tardigrades to a moon-like condition and then measured their survival.

You will have 10 minutes in breakout rooms to analyze the data and answer a few questions about your conclusions and interpretations. Please pick a spokesperson to report back the larger group.

What questions do you have before we begin?

(Unmute & ask, or type in the chat)

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Data Analysis & Interpretation

temperature

radiation

vacuum

impact speed

dehydration

10 minutes in breakout rooms

(group 2)

(group 5)

(group 1)

(group 3)

(group 4)

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temperature

radiation

vacuum

impact speed

dehydration

(group 2)

(group 5)

(group 1)

(group 3)

(group 4)

Report Back & Group Discussion

2 mins per group

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Temperature

  • Moon surface temps: Night -300 °F(-180 °C), Day +225 °F(105 °C)
  • Big temperature swings because there is no atmosphere to trap and distribute heat

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Radiation

  • 0.0015 Gy/d x 365 days = ~0.5 Gy per year on moon’s surface
  • Tardigrades can survive 10,000 x more radiation than humans! (0.5 Gy to 5000 Gy is a difference of 4 orders of magnitude )

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Dehydration

  • As of 2025, the tardigrades crash landed on the moon 6 years ago.
  • Humans can survive without water for 3-4 days.

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Vacuum

  • The pressure on the moon’s surface is 3 x 10⁻¹⁵ bars or 2.96 x 10⁻¹⁵ atmospheres, which is an incredibly low pressure, effectively a near vacuum
  • For reference, humans cannot survive pressures lower than 6180 Pa

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Impact Speed/ Shock Pressure

Humans can experience body damage at 1 Mpa (1 megapascal = 0.001 Gpa)

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Do you think the tardigrades could have survived the crash?

Could they still be alive today?

(Vote in the zoom poll)