a data repository and analysis tool for quantifying metabolism in vivo
Michael Neinast
Lance Parsons
Fan Kang
Robert Leach
John Matese
tracebase.princeton.edu (public)
tracebase-rabinowitz.princeton.edu (private)
designed for infusions
Tracebase is designed for steady-state infusion experiments
designed for infusions
Upload data from any infusion!
From one infusion
From many
Compare anything
Catheterized mice infused with isotope-labeled nutrient
Mass-spectrometry measures compounds and their isotope labeling
.raw
.mzXML
Raw data files
Researchers extract the abundance and labeling of compounds
.xlsx
experiment information
results
Experiment Workflow
TraceBase optimizes data for any analysis.
Consistency
Ready for variety of other tools
PeakData.tsv
PeakGroups.tsv
Fcirc.tsv
What can you do with TraceBase?
Browse experiments and results.
Download -> Compare Data across datasets
~30 minutes in R
Uniform organization -> compare anything
Mike MacArthur
PeakData.tsv
PeakGroups.tsv
Fcirc.tsv
Use PeakData to go deeper
Ratio of M+3 : M+2 labeling in malate from glucose infusions
Use everything to model fluxes
Daniel Weilandt
Mine raw data to find see more compounds.
Xi Xing
.mzXML
download well-organized mzXML
mine data for additional compounds which the original researcher had ignored.
How could TraceBase work outside of our lab?
Rabinowitz Lab
Jang?
Hui?
TeSlaa?
Reynolds?
Bartman?
Public
Private instances of TraceBase hosted by each lab
Public instance for published data only
submitted by anyone
hosted by LSI at Princeton
Arany?
Deberardinis?
Rathmell?