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Ag Biotechnology CDE

Leah Sandall

Dept. of Agronomy and Horticulture

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Ag Biotechnology CDE

  • Written Test: 50 questions
  • Identification Test: 25 items
  • Practicum: Evaluate problem, research, provide conclusions
  • Event Rules and example:

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Practicum

  • Preparation before CDE
  • Google Slides presentation submitted – no oral presentation
  • Computer/tablet for research & Google Slides work

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http://ge.unl.edu

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Genetically Engineered Apples (2020)

  1. Extract All DNA from organism

Apple Tree

2. Isolate (Clone) the one gene of interest

Gene

Browning Enzyme

3. Modify the Gene to Knockout the Browning Enzyme

4. Transform into apple cells

plantlets

5. Propagate (clone) apple plant from cell

6. Breed the transgenic plant with other apple plants/trees

20 pts for each step

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Gene Edited cattle - Cosmo

1. Design the gene using gene editing on cattle DNA to express SRY gene on chromosome 17.

2. Implant transformed

egg in surrogate cow

Gene

(Tulane Univ.)

3. Cross the gene edited bull with other cows

4. Testing to ensure offspring contain SRY gene

30 points for each step (total 120 points); partial points can be given

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Key differences between plant and animal GE

  • Transformation method
    • Gene gun, agrobacterium, pronuclear injection
  • Propagation vs. surrogate
  • Breeding process
  • Creating a transgenic or using gene editing
    • Either plants or animals

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Tips for Punnett Squares when developing breeding plan

  • Identify the targeted end-result genotype
  • Be consistent with placement of male/female gametes in Punnett squares
  • 4-square Punnett squares vs. 1 large Punnett square
    • Max. 3 gene pairs in 1 large Punnett
  • Show steps/work on the slide

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Questions?

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  • Challenges & Successes
  • Examples from the classroom of Biotech learning

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Contact info: Leah Sandall

    • lsandall5@unl.edu, 402-472-9295