pGLO Bacterial Transformation 2021
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1. True or False.  An example of Genetic Transformation is in bioremediation, where bacteria can be genetically transformed with genes to enable them to digest oil spills.
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2.  True or False.   This laboratory will entail transforming genes from the plasmids of bacteria to the genes of Aequorea victoria.
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3.  True or False.  The Bio-Rads unique pGLO plasmid codes for the Fluorescent Protein (GFP), the resistance to the antibiotic ampicillin (bla), and the regulatory gene for the catabolic metabolism of arabinose.
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4.  This lab will employ three steps and they include:   1:  Use a Transformation Solution.  2:  Carry Out heat shock.  3:  Grow the transformed bacteria with nutrients and an antibiotic.
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5.True or False. Using your starter plate of bacteria and a sterile loop 2 - 4 large colonies of bacteria are selected and placed into a +pGLO tube and a  -PGLO tube that contains CaCl2 already.  It is important to select starter colonies to ensure that the bacteria is actively growing.
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6.  True and False. When you examine the pGLO DNA solution it will appear fluorescent green under UV light in step 5, page 8.
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7.  True or False.  You will pipet 10 micro liters (u) of pGLO plasmid in both the +pGLO and -pGlO tube.
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8.  True or False.  Both the +pGLO and -pGLO tubes are Heat shocked by placing them in ice, then in a water bath for 50 seconds, and then back in ice quickly.
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9.  True or False. The control suspension is the tube labeled +pGLO.
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10.  True or False.  The only plate that should have bacteria that expresses the GFP in the presence of UV light will be the plate with +pGLO LB/amp/ara
LB = nutrient broth - food for bacteria ,     amp = ampicillin - antibiotic,     ara = arabinose = sugar     Read Page 2!
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