Nematode Identification and Quantification

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Whole Nematode Community Analysis: nematodes will be separated from soil, counted under the microscope and a subset of nematodes (including beneficial bacterial feeding, fungal feeding, omnivorous, predatory and plant parasitic groups) identified to genus for subsequent calculation of soil health indices.

Plant Parasitic Nematode Analysis: nematodes are separated from soil using the sugar centrifugation method, counted under the microscope and identified to genus.

Plant Parasitic Nematode Quantification by Real Time PCR (qPCR): nematodes will be separated from soil, after which their DNA will be extracted and numbers determined using qPCR with species specific primers. This method is only available for ring nematode (Mesocriconema xenoplax), lesion nematode (Pratylenchus vulnus) and root knot nematodes (Meloidogyne incognita). We can also determine species for other plant parastitic nematodes using other molecular methods (email for details).

Name *

Company or Affiliation

Are you a part of the UC/UCANR system or have you already completed the necessary paperwork to have your samples accepted? *

Yes - you may continue with the rest of the form

No - please contact the lab before sending samples

Phone Number and Email Address *

Shipping Address *

Types of Analysis *

Community

Pest

Molecular

Required

Number of Samples *

County of Origin (samples can only be received from within CA) *

Brief Description of the problem including previous, current, and future crop types *

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