SEQUENCING SUBMISSION FORM
Please see our FAQ at
Agree on our Terms?
Please read our Terms and Instructions here
Yes it is reasonable, I agree. I will submit neatly labeled samples in permanent dark ink and large font and all the names will follow the pattern you require (SJ001, SJ002, SJ003, …)
Affiliation of the PI who owns the account used to pay for the sequencing services. Columbia University PI account are charged directly by Inter Departmental Invoice using their chartstring (next page). HICCC members must be listed at
and DSB at
DEPARTMENT OF SYSTEMS BIOLOGY AND/OR HERBERT IRVING COMPREHENSIVE CANCER CENTER
First Last. This is the point of contact during the sequencing
RESEARCHER DIRECT PHONE NUMBER
YOUR ADMIN OR ACCOUNT PAYABLES FIRST LAST NAME
This is the person to contact to process the payment
Please enter the ticket number if you have discussed this submission with us (the ticket number can be found in the header of the ticket). Please do not enter the quote number here, nor a random number. If you do not have a ticket number, please just leave this answer blank. If you do have a ticket, please answer, that's useful to us.
This is the genome to map your sample against. One genome type per order: if you have 5 mouse samples and 5 human samples, you will need 2 orders.
MOUSE HUMAN HETEROKARYON
MARINE ALGAL COMMUNITY
Number of samples submitted
Packages 120, 131-132, 401-402, 421, and 431 need pre-approval. Packages 133-134 and exomes packages 201-202 need approval IF you submit less than 6 samples. Please email
, and please CC the PI in the request. Once we approved, please enter the ticket number in the TICKET field above. Please see price list at
120 - RNA STRDPOLYA 15M SE100
121 - RNA STRDPOLYA 30M SE100
122 - RNA STRDPOLYA 30M PE100
123 - RNA STRDPOLYA 60M PE100
124 - RNA STRDPOLYA 90M PE100
131 - RNA RIBOZERO 30M SE100
132 - RNA RIBOZERO 30M PE100
133 - RNA RIBOZERO 60M PE100
134 - RNA RIBOZERO 90M PE100
141 - RNA CLONTECH 30M SE100
231 - EXOME XGEN 50X 25M PE100
232 - EXOME XGEN 100X 50M PE100
301 - GENOME TRUSEQNANO 15X 225M PE100
302 - GENOME TRUSEQNANO 30X 450M PE100
401 - LIBRARY SELF 300M SE100
402 - LIBRARY SELF 300M PE100
411 - LIBRARY SELFUSE NEXTSEQ
421 - LIBRARY SELF 270M PE125
431 - LIBRARY PLATESEQ 300M PE25X50
95% of our users follow this step well: In order to avoid any confusion, all samples must be labeled clearly. If your first name Joe and your PI's first name is Susan, please name your samples SJ001, SJ002, SJ003, … No two projects from the same customer should have identical sample identification numbers. Please ensure that each of your projects uses never-used, unique ID's.
You must provide a Bioanalyzer report if you submit RNA, cDNA or self-libraries. CUMC users should use the Molecular Pathology service at ICRC Room 301
I WILL PROVIDE A BIOANALYZER REPORT, ALL RIN ARE > 8
Please give the concentration of sample from the bioanalyzer report. Any gel photos for DNA can be emailed to
Over the past year, our average turnaround time for RNA-seq has been 25 days from the time we receive your samples and they are ready for sequencing, and the time we deliver the data. If you need anything urgently, please submit a request for approval for EXPRESS first by writing at
. Express requests are not eligible for volume discounts. PI approval is required, if you are not the PI, please CC your PI in the request.
HOW TO RELEASE
PLEASE PUT A LINK ONLINE ON YOUR WEBSITE
COPY ON HARD DISK - I WILL PICKUP AND RETURN THE DISK
TRANSFER TO MY C2B2 ACCOUNT (C2B2 MEMBERS OR COLLABORATORS ONLY)
RELEASE EMAILS CC
In addition to the PI and the researcher, who should receive the email when we release the data. Separate multiple with commas.
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