生化所生物物理設施Cuvette或物品借出與歸還線上登記
一、借用辦法與規則(使用者委員會20170628通過):
1. 借用設施比色管前後需登記,使用後務必清理乾淨。
2. 使用前後需與管理者當面點交,如未當面清點,事後發現任何汙損將由最後一位登記使用者負責。
3. 借用期限以一周為限,不得私下轉借他人使用。
4. 如有發現未清洗乾淨或破損現象,除違規處分外,需重新清洗或購買同款物品更換。
5. 不得使用放射性、高揮發性、腐蝕性及感染性樣品。
6. 每支比色管視為一個個案,違規將得以累計處罰。例如:借用三支比色管,三支都未清洗乾淨視為違規三次。
7. 違反規定,第一次:停權一個月,通知所屬PI並按第4點處置
       第二次:停權三個月,通知所屬PI並按第4點處置
       第三次:永久停權,通知所屬PI並按第4點處置
二、比色管清洗方法參考:
Removing your sample/precipitates and rinse by ddH2O several times.
1. Acid wash (wear gloves and do this in the chemical hood): Don’t use NaOH or strong bases!
(1) Prepare 2M HCl or HNO3. (If the cuvette still dirty (can see something on the wall)
or the water blank has unusually absorbance, try 50% H2SO4!)
(2) Add acid with glass dropper to the top, cover with parafilm, and invert to mix carefully.
(3) Let the cuvette sit for at least 1 hour.
(4) Remove acid and wash by ddH2O.
(5) After washing away acid, use methanol/ethanol/acetone to rinse the cuvette and air dry.

2. Tergazyme (Enzyme-Active Powdered Detergent):
(1) Make a fresh 1% Tergazyme solution in ddH2O.
(2) Add 1% Tergazyme solution to the cuvette and incubate at RT or 50 degree for 1 hour (do not exceed 55 degree).
(3) Remove the solution and wash by ddH2O.
(4) Use methanol/ethanol/acetone to rinse the cuvette and air dry.

判定方式: 光譜儀測空cuvette的訊號需低背景值及無吸收訊號 (The clean cuvette must has low background and no absorption)!
Example: 1mm CD cuvette scanning by CD (260-195nm, DIT 1sec, slit 1sec, scanning speed 50 nm/min), the signal should be flat and less than +/-0.5 mdeg.
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