1. What are the approximate dimensions of a 20S proteasome particle in the following micrograph?
Keep in mind the physical dimensions of your particle!
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2. Which of the following problems could arise if a particle is extracted with too small of a box size? Select all that apply.
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3. Which of the following extraction boxes would be most appropriate for the following particle?
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4. Downsampling is the process by which the data in extracted particles is compressed into more computationally tractable formats. In which of the following scenarios would downsampling be most appropriate? Select all that apply.
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5. What factors should you consider when deciding on the number of 2D classes? Select all that apply.
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6. Which of the following are potential consequences of utilizing too few 2D classes? Select all that apply.
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7. What is the purpose of a circular mask in 2D classification? Select all that apply.
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8. From the array of class averages shown below, select the classes that include obvious ice, aggregates, or junk.
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9. From the array of class averages shown below, select the classes that appear to contain adjacent particles within the mask
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10. From the array of class averages shown below, select the 'best' looking classes to be used in subsequent data processing steps.