Cyto Conference 2019 Workshop 1: Creating a Repository of Best-Practice Guidelines for Successful Cell Sorting Outcomes
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Situation: You are given a new electrostatic cell sorter. The sorter is able to distinguish different cellular populations with varying fluorescence and scatter intensities. You now need to evaluate how well the sorter sorts cells for downstream live cell experiments.
What variables would you consider for successful sorting outcomes? (sheath type, sheath pressure, nozzle size, collection media, temperature, etc)
What cell sorting variables do you deem important that OTHERS report about their cell sorters and cell sorting experiments?
How do you evaluate the success of a cell sorting experiment?
What methods do you use to determine the success of a cell sorting experiment?
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Thank you very much!
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