Lab Skills 2.2 - Liquid transfer

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Lab Skills 2.2 - Liquid transfer - advanced

Quiz for module Lab Skills 2.2 - Liquid transfer - advanced (LS_2.2_v2)

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zID *

Module Document Declaration

This module is based on the following documents which you must have read, and declared as read, and the following videos which you must declare as viewed:

SCI-CHEMSCI-SWP-6889 - Liquid transfer D – Reusable syringes and needles
SCI-CHEMSCI-SWP-6921 - Liquid transfer E – Adjustable and fixed volume pipettes

PLEASE NOTE:
-> The declaration statement has no bearing on your quiz score.
-> If you have declared the documents as read during a previous quiz attempt, you do not need to declare them as read again.
-> If you have passed the quiz previously but not declared the documents as read, you may declare them as read at any time but you do not need to pass the quiz again.

I declare...

… that I have read the following required documents, and viewed the following required videos (if applicable) for this module and will comply with the instructions and procedures contained within them. (Select all that apply)

SCI-CHEMSCI-SWP-6889

SCI-CHEMSCI-SWP-6921

Module Quiz

What are the four most important rules when using reusable glass syringes? (Select all that apply) *

Always use thin (~27 gauge) needles for transferring liquids

Never interchange plungers and barrels

Always clean reusable syringes and needles immediately after use

Choose the right syringe and needle for your application

Take extra care to avoid needle stick injuries

Always use syringes in a fume cupboard

Always use luer slip fittings

Required

Which type of reusable glass syringe is preferred for use with halogenated solvents? *

Cemented Needle (CN)

Removable Needle (RN)

Which sizes of needles are preferred for transferring liquids? *

Thicker needles (approximately 14 – 21 gauge)

Thinner needles (approximately 23 – 27 gauge)

Extra care must be taken with reusable needles because: (select all that apply) *

If the needles become blocked then the entire syringe will be unusable

They do not come with a safety cap in place

They may be extra-long and require more clear space to work in

Required

Extra care must be taken with cemented needles because: (select all that apply) *

If the needles become blocked then the entire syringe will be unusable

They may be extra-long and require more clear space to work in

They do not come with a safety cap in place

Required

True or false – the preferred method for drying a glass syringe is to place it in a vacuum desiccator at room temperature for at least overnight. *

True

False

True or false – you must wet a PTFE tipped plunger in solvent prior to inserting it into the barrel. *

True

False

True or false – you should prepare cleaning solutions and a waste flask BEFORE you use the syringe to transfer your solution. *

True

False

When using a glass syringe and needle to transfer liquid from an enclosed vessel (for example, a chemical bottle sealed with a septum), you should: (select all that apply) *

Account for pressure changes in the vessel by using another needle connected to the Schlenk line or injecting inert gas before withdrawing the solution

Secure the vessel with a clamp if necessary so that you don’t place your hand in the path of the needle

Allow the pressure to equalise in the syringe before removing it from the vessel

Tidy the work area and ensure other people or equipment will not encroach on your work space

Required

Which is the proper method for cleaning a reusable glass syringe? *

After you finish setting up your experiment, you must rinse with an initial pure cleaning solvent that is known to solubilise your solution, followed by rinses with pure miscible solvents and finally pure acetone and drying at room temperature.

Immediately after dispensing your solution, you must rinse with an initial pure cleaning solvent that is known to solubilise your solution, followed by rinses with pure miscible solvents and finally pure acetone and drying at room temperature.

Which cleaning solutions should you never use for glass syringes? (Select all that apply) *

Acetone

Detergents

Base bath

Water

Ethanol

Required

True or false – needles must always be disposed of in an approved Sharps Container. *

True

False

True or false – syringe barrels and plungers must always be disposed of in an approved Sharps Container. *

True

False

True or false – you can adjust the volume of an Eppendorf pipette outside the limits as long as it’s only by a few microliters. *

True

False

Which of the following solutions MUST NOT BE USED with Eppendorf pipettes? (Select all that apply) *

Dichloromethane

Toluene

Acetone

1% SDS in water

90% acetic acid

50% ethanol in water

20% ethanol in DMSO

Tween 20 (pure)

Mineral oil

Concentrated nitric acid

20% toluene in ethanol

20% DMSO in water

10% ammonia solution

Required

Which of the following solutions CAN BE USED with Eppendorf pipettes? (Select all that apply) *

1% SDS in water

20% DMSO in water

Tween 20 (pure)

10% ammonia solution

Acetone

Dichloromethane

50% ethanol in water

Toluene

Mineral oil

Concentrated nitric acid

20% toluene in ethanol

20% ethanol in DMSO

90% acetic acid

Required

True or false – you must take care not to allow the solution to contact the pipette or enter the pipette chamber, and you must immediately clean up any spills or points of contact with the solutions to minimise the exposure time between the pipette and the chemical. *

True

False

When using an adjustable pipette to transfer liquid, you must: (select all that apply) *

Not lay the pipette down if there is liquid in the tip

Not exceed the minimum or maximum volume limits for the pipette

Return the pipette to its station immediately after use

Draw in and dispense the liquid slowly and completely

Use an approved Sharps Container to dispose of the used plastic tips

Required

True or false – tips that are contaminated with a hazardous substance must be disposed of as contaminated solids. *

True

False

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