2E Quality control of isolated DNA

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Quiz related to the exercise on interpretation of quality control results from Qubit and NanoDrop.

Course: "Whole Genome Sequencing workflow- from isolate to analysis"

Which of the following sample combinations have an unacceptable 260/280 ratio?

Please refer to the images provided in the exercise power point on slides 3 and 4.

1 point

A & C

A & D

D & B

A, C & D

Which of the following sample combinations have acceptable 260/280 ratios?

Please refer to the images provided in the exercise power point on slides 6 and 7.

1 point

A & B

A, C & D

A & C

A, B, C & D

Which of the following sample ID combinations have unacceptable 260/230 ratios?

Please refer to the table on slide 8 of the exercise power point.

1 point

1, 3, 5, 7, 9, 10

2, 4, 6, 8, 9, 10

3, 4, 5, 7, 8, 10

2, 3, 7, 8, 9, 10

Provide two sample IDs that have the highest DNA concentration (ng/ul) readings.

Please refer to the table on slide 9 of the exercise power point

1 point

2 and 4

8 and 10

4 and 5

6 and 7

Tick all statements that are correct for the NanoDrop Spectrophotometer

1 point

It uses UV absorbance measurements

It uses X-ray absorbance measurements

The 260/280 ratio determines protein contamination of nucleic acid

The 260/280 ratio should be below 1.8 for good pure quality DNA

The 260/280 ratio should be above 2.1 for good pure quality DNA

The 260/280 ratio should be between 1.8 and 2.1 for good pure quality DNA

It can distinguish between the measurement of DNA, RNA and protein

The 260/230 ratio determines RNA contamination

The 260/230 ratio determines organic contamination

The 260/230 ratio should be between 1.8 and 2.2

The 260/230 ratio should be between 2.1 and 2.5

Required

Why is the Qubit often preferred over the Nanodrop for quantification of DNA for sequencing?

1 point

It quantifies all proteins

It quantifies all carbohydrates

It measures the concentration of intact dsDNA

What advantage has the Qubit fluorometer over the Nanodrop?

1 point

The Qubit is more sensitive in measuring dsDNA

The Qubit is more accurate in measuring dsDNA

All of the above

The Qubit fluorometer makes use of UV rays

1 point

True

False

In using the Qubit, the fluorescent dye in the working solution binds to the target material (i.e. the dsDNA)

1 point

True

False

What is the use of the photodetector in using the Qubit?

1 point

Detects the UV radiation

Detects the temperature in the lab

Detects the fluorescent signals

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