Quiz 9
Answer all the questions below and choose the best answers. For some questions, there may be more than one correct answer.
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What are the key problems of using microplates in high-throughput screening? *
10 points
Required
When doing a spectroscopic measurement using microplates, what are the key considerations? *
10 points
Required
What are the strategies for reducing the evaporation problem of microplates? *
10 points
Required
Which of the following chemicals is used for cell disruption or permeabilization? *
10 points
Required
Abayomi Johnson is developing a high-throughput screening system for his enzyme. The three internal controls of his assay plate gave the absorbance values of 0.567, 0.534 and 0.611. Calculate the mean of the internal controls. *
10 points
Required
Using the information provided in the previous question, calculate the standard deviation of the internal controls. *
10 points
Required
Using the information provided in the previous question, calculate the coefficient of variation (CV) of the internal controls. *
10 points
Required
Enzymatic assay should be conducted under what condition? *
10 points
Required
The reaction rate constants for an enzymatic reaction that obeys the Michaelis-Menten model are k1 = 1.50 μM-1s-1, k-1 = 7.50 s-1, and k2 = 150 s-1. Calculate the Km value. *
10 points
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Using the information above, calculate the Vmax value, if the enzyme concentration is 10 nM. *
10 points
Required
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