Common Calculations Encountered in the Molecular Biology Lab

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Input a number. Do NOT include units. Round all of your answers to the first decimal place (i.e. 3.4) or whole number (ie. 5 NOT 5.0). For large numers do NOT use scientific notation nor should you include commas. Numbers < 1 should include a 0 before the decimal place (i.e. 0.6). This "test" will auto grade. Therefore, typos will be graded as incorrect.

10 micromolar is equivalent to ___________ nanomolar.

1 nanomolar is equivalent to ______1000____ picomolar.

Your Quality Assurance Document says you have precisely 34 nmols of oligo as a dry pellet. How much DNA grade water (in ul) should you add to the dry pellet to create a 100 uM solution?

Your Quality Assurance Document says you have precisely 60 nmols of oligo as a dry pellet. How much DNA grade water (in ul) should you add to the dry pellet to create a 80 uM solution?

How much water (in ul) should you add to 1 ul of plasmid DNA (540 ng/ul) so that the final concentration is 25 ng/ul?

How much water (in ul) should you add to 1 ul of plasmid DNA (634 ng/ul) so that the final concentration is 15 ng/ul?

How much water (in ul) should you add to 1 ul of plasmid DNA (854 ng/ul) so that the final concentration is 150 ng/ul?

You digest 500 ng of DNA. You expect one band to be 6 kb in length and the other band to be 3 kb in length. How much DNA (in ng) will be present in the fragment that migrates to the 6 kb position

You digest 1000 ng of DNA. You expect one band to be 345 bp in length and the other band to be 655 bp in length. How much DNA (in ng) will be present in the fragment that migrates to the 655 bp position

You digest 1500 ng of DNA. You expect one band to be 1.215 kb in length and the other band to be 513 bp in length. How much DNA (in ng) will be present in the fragment that migrates to the 513 bp position

Let's say that your restriction enzyme digest requires 30 U of Eag I for a single 1 hour reaction. How much less enzyme can you add (in Units) for a 16 hour digest? Consult the NEB catalog.

Let's say that your restriction enzyme digest requires 20 U of AvaI I for a single 1 hour reaction. How much less enzyme can you add (in Units) for a 16 hour digest? Consult the NEB catalog.

Let's say that your restriction enzyme digest requires 10 U of Cla I for a single 1 hour reaction. How much less enzyme can you add (in Units) for a 16 hour digest? Consult the NEB catalog.

You want to load your 20 ul restriction digest onto a gel. How much 6X loading dye should you add to the digest to achieve a final concentration of 1X (in ul)?

You want to load your 30 ul restriction digest onto a gel. How much 6X loading dye should you add to the digest to achieve a final concentration of 1X (in ul)?

You want to load your 40 ul restriction digest onto a gel. How much 10X loading dye should you add to the digest to achieve a final concentration of 1X (in ul)?

You want to load your 50 ul restriction digest onto a gel. How much 10X loading dye should you add to the digest to achieve a final concentration of 1X (in ul)?

You need to add 25 Units of Spe I to your 20 ul enzyme digest reaction. Spe I is at a concentration of 10,000 units / ml. How much enzyme should you add (in ul)?

You need to add 10 Units of Hind III to your 20 ul enzyme digest reaction. Hind III is at a concentration of 20,000 units / ml. How much enzyme should you add (in ul)?

You need to add 15 Units of Avr II to your 30 ul enzyme digest reaction. Avr II is at a concentration of 5,000 units / ml. How much enzyme should you add (in ul)?

Your plasmid DNA is at a concentration of 345 ng/ul. You want to digest 1 ug. How much will you need (in ul)?

Your plasmid DNA is at a concentration of 450 ng/ul. You want to digest 1.5 ug. How much will you need (in ul)?

Your plasmid DNA is at a concentration of 650 ng/ul. You want to digest 500 ng. How much will you need (in ul)?

You have a 12 kb plasmid that you digest with Hind III. If the plasmid is correct, you expect to see a 300 bp fragment on a gel. What is the minimum amount of DNA (in ng) that you should digest to be able to visualize this fragment on a gel (assuming 20 ng is sufficient for visualization)?

You have a 10.3 kb plasmid that you digest with Hind III. If the plasmid is correct, you expect to see a 250 bp fragment on a gel. What is the minimum amount of DNA (in ng) that you should digest to be able to visualize this fragment on a gel (assuming 20 ng is sufficient for visualization)?

You have a 9.6 kb plasmid that you digest with Hind III. If the plasmid is correct, you expect to see a 450 bp fragment on a gel. What is the minimum amount of DNA (in ng) that you should digest to be able to visualize this fragment on a gel (assuming 20 ng is sufficient for visualization)?

How much NaCl powder (in g) should you add to make 1 Liter of a 5 M solution?

How much MgCl2 powder (in g) should you add to make 100 mls of a 1 M solution?

How much EDTA powder should you add (in g) to make 50 mls of a 0.5 M solution?

You want to prepare a 2% agarose gel (total volume = 30 mls). How much agarose should you add (in g)?

You want to prepare a 0.8% agarose gel (total volume = 50 mls). How much agarose should you add (in g)?

You want to prepare a 3 % agarose gel (total volume = 75 mls). How much agarose should you add (in g)?

You have 0.5 g of X gal in a large bottle. How much solvent should you add (in mls) to the bottle to create a 2% solution?

You have 0.5 g of cholesterol in a large bottle. How much solvent (in mls) should you add to the bottle to create a 5 mg/ml solution?

You have a 500 mM stock solution of magnesium chloride. Your reaction mix calls for a final concentration of 25 mM. Your total reaction volume will be 50 ul. How much magnesium chloride stock solution should you add to the reaction mix (in ul)?

You have a 10 X Reaction Buffer, Your reaction mix calls for a 1X final concentration. Your total reaction volume will be 25 ul. How much 10X buffer should you add to the reaction mix (in ul)?

Your forward primer is at a concentration of 100 uM. Your PCR reaction calls for a final concentration of 1.2 uM. Your total reaction volume will be 50 ul. How much forward primer should you add to the PCR reaction (in ul)?

Your forward primer is at a concentration of 25 uM. Your PCR reaction calls for a final concentration of 1.5 uM. Your total reaction volume will be 30 ul. How much forward primer should you add to the PCR reaction (in ul)?

You have a 1 M stock solution of IPTG. You need 10 mls of a 50 mM working solution. How much of the stock solution will you add (in mls) to your diluent?

You have a 5 M stock solution of NaCl. You need 300 mls of a 25 mM working solution. How much of the stock solution should you add (in mls) to your diluent?

You have a 10 % solution of SDS (sodium dodecyl sulfate). What is its concentration in mg/ml?

You have a 5 % solution of ethidium bromide. What is its concentration in mg/ml?

You have a 1 % solution of X-gal. What is its concentration in mg/ml?

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