iGEM Athens 2021-Survey
Introduction

We, in iGEM Athens propose a novel biological system that can produce, de novo, dNTPs and the Pfu DNA polymerase in order to solve the shortage of these PCR ingredients. The key point of our proposed solution is based on taking advantage of the natural ways organisms produce, de novo, dNTPs, as it is essential for their reproduction states. This is based on enzymatic reactions that are common in all organisms, and include the ribonucleotide reductase (RNR) and Thymidylate synthase (TSase) enzymes. The synergetic action of both enzymes has as a result the conversion of NTPs to dNTPs, which are essential for the production of DNA. These enzymes and Pfu polymerase will be overexpressed after their insertion with a plasmid. We hope that the mentioned system will provide to a wide range of different laboratories accessibility to techniques that use these reagents but also make diagnostics more affordable and easier to accomplish.

Goal

The following questions will help us direct our project to the needs of the research community. According to your answers we will collect data to ensure our project provides with a solution to issues that are present in laboratories all around the globe.

Estimated time for completion: 5 min
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What kind of lab are you currently working in? *
dNTPs
1. Do you use dNTPs for your experiments? *
2. How important are dNTPs for your lab experiments? *
Not important
Very important
3. In which techniques do you use them? *
4. Do these techniques require skilled personnel? *
5. How difficult is the correct storage and handling of dNTPs in your lab? *
Not difficult
Very difficult
6. How often do you get dNTPs for your lab? *
7. What is the usual quantity of dNTPs that you order? *
8. During the pandemic over the past year, has the quantity of dNTPs your lab uses, risen? *
9. Was there any problem with the availability of dNTPs? *
10. Based on your annual budget how expensive are dNTPs? *
Not very expensive
Very expensive
11. How important is the purity of dNTPs in your experiments? *
Not important
Very important
12. How informed do you believe you are on the current industrial production and purification processes of dNTPs? *
Not at all
Very well informed
13. How attractive do you find the idea of producing dNTPs in your lab using a streamlined and eco-friendly protocol? *
Not interested
Very interested
14. How attractive do you find the idea of using dNTPs that are stored in a lyophilized lysate of E.coli cells (which allows it to be stored in room temperature) ? *
Not interested
Very interested
15. From question 13 and 14, which one do you find most attractive? *
16. Would you prefer the extraction of dNTPs to be done with slower methods, such as diffusion, over lysating the bacterial culture ? *
DNA polymerase
1. Do you need DNA polymerase for your experiments? *
2. Which DNA polymerase do you use in your experiments? *
3. Why do you use this particular DNA polymerase? *
4. How important is DNA polymerase for your lab? *
Not important
Very important
5. How often do you get DNA polymerase for your lab? *
6. How attractive do you find the idea of producing DNA polymerase in your lab using a streamlined and eco-friendly protocol? *
Not interested
Very interested
7. How important is the purity of DNA polymerase in your lab protocols? *
Not important
Very important
Would you like to add anything else?
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