Spring 2019 ORFan Gene Project Pre-Test
Thank you for participating in the ORFan Gene Project Assessment. Participation in this pre-test is completely voluntary. Your individual score will be kept confidential, and will not be revealed to your instructor. Furthermore, your performance will not be used as part of your grade for the course in which this assessment is being administered. The ORFan Gene Project will use aggregate data from this assessment instrument in order to determine the effectiveness of our work as a whole, to make improvements going forward, and to publish our findings. Thus, your participation is incredibly important to our work, and we thank you again, in advance. If you have any questions or concerns, please contact Jill Keeney at Keeney@juniata.edu.

Please answer each question below to the best of your ability. Since we are assessing your knowledge, rather than your guessing ability, please do use answer (e) "I do not have the knowledge base to answer this question" if that is true.

Academic Institution *
Your answer
ID Number: Your unique identifier will be the following nine digits: Your birth month (xx) birth day (xx) and zip code (XXXXX). So, if you were born on June 19 and your zipcode is 16666, your ID would be 061916666. *
Your answer
Demographics (optional)
Please indicate your gender
Please indicate your Ethnicity origin (or Race): Please specify your ethnicity, checking as many as apply.
I Certify that I am at least 18 years old *
Column 1
Knowledge and Skills Questions
Which of the following is NOT a gene ontology domain? *
Which of the following best qualifies as a molecular function? *
A(n)_____ ORF is likely to encode an expressed protein, but there is a lack of specific experimental data demonstrating protein production. *
A gene of unknown function (ORFan) is best defined as a gene for which: *
Is this statement true? "The ORFs of unknown function in yeast are easily identified as such ORFs only have a systematic name (e.g. - YFL039C) and no standard name (e.g. – ACT1)" *
Which of the following is best suited to comparing protein sequences? *
Which of the following sequences is best suited for BLAST-N? *
Which of the following tools would you use to determine the transcription expression profile for an ORF? *
YLR042 is a verified ORF of interest. SGD description reads: "Cell wall protein of unknown function; localizes to the cytoplasm; deletion improves xylose fermentation in industrially engineered strains; YLR042C is not an essential gene" (Note: xylose is a sugar). Which of the following tools would be best to use to investigate the discrepancy in the description that states this protein is a cell wall protein and that it is located in the cytoplasm? *
After subjecting the sequence for your favorite protein to a database search, you are provided with several related proteins. With respect to the e-values for the related proteins, which of the following is most likely to be true? *
You have received the aligned sequences for two proteins. Which of the following would be evidence that they could share a similar function? *
Which of the following is the most general term used to describe evolutionarily related genes? *
if an ORF of unknown function has homology only to genes in other yeast species, is this gene of interest to scientists? *
Research Skills Questions
Please look over this inventory of elements that might be included in a course. For each element, give an estimate of your current level of ability. Please use this scale: mark a "1" if you have no experience or you feel inexperienced, mark a "2" if you have some experience, or mark a "3" if you have much experience or feel you have mastered this element. Your current level of ability may be a result of courses in high school or college, or it may be a result of other experiences such as jobs or special programs. *
1 - no experience or feel inexperienced
2 - some experience
3 - much experience or mastered this element
A lab or project where no one knows the outcome
A project in which students have some input into the research process and/or what is being studied
A project entirely of student design
Become responsible for a part of the project
Read primary scientific literature
Collect data
Analyze data
Present results (orally, written papers or reports, posters)
Computer modeling
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