Reference sequence databases for global zooplankton biodiversity: Optimization, applications and user guidelines
OVERVIEW
Molecular genetic approaches are used with increasing frequency to detect, discriminate, and identify species of marine zooplankton, and to characterize diversity of marine ecosystems. We have progressed rapidly from using DNA barcodes to identify single specimens, to DNA metabarcoding to characterize diversity of environmental samples and communities. In both cases, assignment of a sequence to a species, or classification to a taxonomic group, requires an accurate and complete reference database of DNA sequences based upon morphologically identified specimens. Currently, zooplankton sequence data can be found in several databases, which differ in their submission process, associated metadata, availability of sequence data (local vs. open-access) and taxonomic groups represented. In addition, there are a variety of available algorithms to assign the sequences to diverse taxonomic ranks.
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