Amino Labs intro to PCR quiz

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After going through the PCR power point, test your understanding with this quiz.

The extremophilic bacteria Thermus Aquaticus (Taq) was found in Yellow Stone National Park. Why did the discovery of Taq enable PCR? *

1 point

Taq polymerase has an additional binding domain allowing it to copy two DNA strand simultaneously.

Taq polymerase does not require a buffer to copy DNA

Taq polymerase is a larger enzyme than other polymerases making it easier to purify.

Taq polymerase can withstand very high temperatures

What does PCR stand for? *

1 point

Polymeric Chain Reassembly

Peptide Chain Reaction

Protein Chain Reaction

Polymerase Chain Reaction

Which of the following answers is FALSE concerning Taq polymerase.

1 point

This enzyme was the first to be used in PCR.

Optimal activity for this enzyme is around 70 degrees Celsius.

Taq polymerase has advanced proof reading activity.

The error rate for this enzyme is approximately 1 in 20, 000 nucleotides.

Clear selection

Select all the components needed to run a polymerase chain reaction.

1 point

Buffer

Calcium Carbonate

Ribonucleotides (A, U, G, C)

Deoxyribonucleotides (A, T, G, C)

DNA Template

DNA Polymerase

DNA Primers (forward and Reverse)

What are the main chemical activities that occur in a PCR reaction thanks to the thermocyler?

1 point

Denaturing, annealing, and extension

Denaturing, mitosis, and annealing

Decomposition, annealing, and extension

Denaturing, annealing, and translation

Clear selection

What type of bonds break in the denaturing phase of PCR that cause the DNA strands to separate?

1 point

Peptide Bonds

Ionic Bonds

Hydrogen Bonds

Electrostatic Bonds

Clear selection

If you had one double stranded molecule of DNA, how many double stranded molecules would you have after 3 cycles of PCR? (It may help to draw this out)

1 point

Three double stranded DNA molecules

Twelve double stranded DNA molecules

Fifteen double stranded DNA molecules

Sixteen double stranded DNA molecules

Clear selection

Before the polymerase enzyme binds to the template DNA, what must bind first?

1 point

Nucleotides

Primers

The polymerase can bind immediately

Magnesium and Calcium ions

Clear selection

Which of the following options best describes the phases of PCR

1 point

Step 1. Pre-melting, Step 2. Main cycling (30-35X cycles) Step 3. Final Extension

Step 1. Pre-melting, Step 2. Main Cycling (60-65X cycles), Step 3. Final Extension

Step 1. Pre-melting, Step 2. Annealing and denaturing, Step 3. Final Extension

Step 1. Pre-melting, Step 2. Main Cycling (30-35X cycles), Step 3. Final denaturation

Clear selection

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