European Xenopus Resource Needs Survey 2022

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Ghent, July 2022

My lab finds the EXRC: *

The EXRC supports the principles of the 3Rs (Replacement, Reduction and Refinement) for performing more humane animal research: *

The current commercially available X. laevis are (if applicable): *

The current commercially available X. tropicalis are (if applicable): *

Which of the following resources from EXRC do you use and how important are they for successful research in your lab (X. laevis wild type strain):

Not at all

Moderately important

Essential

X. laevis females

X. laevis males

X. laevis fresh testes

X. laevis frozen sperm

X. laevis oocytes

X. laevis embryos

X. laevis egg extracts

Clear selection

Which of the following resources from EXRC do you use and how important are they for successful research in your lab (X. tropicalis wild type strain):

Not at all

Moderately important

Essential

X. tropicalis females

X. tropicalis males

X. tropicalis frozen sperm

X. tropicalis oocytes

X. tropicalis embryos

X. tropicalis egg extracts

Clear selection

Which of the following resources from EXRC do you use and how important are they for successful research in your lab (X. laevis genetically altered lines):

Not at all

Moderately important

Essential

X. laevis females

X. laevis males

X. laevis fresh testes

X. laevis frozen sperm

X. laevis oocytes

X. laevis embryos

X. laevis egg extracts

Clear selection

Which of the following resources from EXRC do you use and how important are they for successful research in your lab (X. tropicalis genetically altered lines):

Not at all

Moderately important

Essential

X. tropicalis females

X. tropicalis males

X. tropicalis frozen sperm

X. tropicalis oocytes

X. tropicalis embryos

Clear selection

Which of the following resources from EXRC do you use and how important are they for successful research in your lab (Molecular resources):

Not at all

Moderately important

Essential

X. laevis in situ probes or expression plasmids

X. tropicalis in situ probes or expression plasmids

X. laevis BACs

X. tropicalis fosmids

Antibodies

pTransgenesis plasmids

X. laevis ORFeome

X. tropicalis ORFeome

Clear selection

Which of the following resources from EXRC do you use and how important are they for successful research in your lab (Support services):

Not at all

Moderately important

Essential

Informal support for frog welfare

Informal support for methods and reagent use

Formal training for legislative requirements (ASPA)

Informal training for animal care

Research Hotel

Clear selection

Which future options for the development of the EXRC would you use:

Yes

Increased biosecurity of animals for supply to the community

Make X. tropicalis transgenic lines fluorescently labelled in major, experimentally targeted cell types including for analysing human diseases

*Make signalling reporter lines (in which/both species?)

Run specific courses on gene editing with the opportunity to target your genes

Growing lines of genetically altered frogs you have produced

Research hotel sessions for using EXRC’s X. tropicalis

Research to improve X. tropicalis performance

Provide Xenopus cell lines

Work to ensure all new transgenic lines are safely stored and available

Yes

Increased biosecurity of animals for supply to the community

Make X. tropicalis transgenic lines fluorescently labelled in major, experimentally targeted cell types including for analysing human diseases

*Make signalling reporter lines (in which/both species?)

Run specific courses on gene editing with the opportunity to target your genes

Growing lines of genetically altered frogs you have produced

Research hotel sessions for using EXRC’s X. tropicalis

Research to improve X. tropicalis performance

Provide Xenopus cell lines

Work to ensure all new transgenic lines are safely stored and available

Clear selection

*If answered YES to the above question, please incidcate: in which/both species?

Things I would like the EXRC to do or to improve:

Thank you for your input.

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