Title: AMD Cell Therapy Efficacy Assessment Using Artificial Intelligence-Based Multi-Spectral Imaging
Speaker: Nathan A. Hotaling, PhDUnit on Ocular and Stem Cell Translational Research, National Eye Institute, National Institutes of Health
I will first present work showing that the biochemical and physiological function of induced pluripotent stem cell derived RPE (iPSC-RPE) can be predicted using novel multi-spectral imaging, machine learning algorithms, and convolutional neural networks (CNN). Then, I will show that this methodology was able to be used on iPSC-RPE derived from patients with dry AMD that were differentiated using an FDA compliant good laboratory practices (GLP) protocol. When combined, the imaging and computational algorithms were able to predict cell function with high sensitivity and specificity as well as classify iPSC-RPE identity to an equal degree as that of traditional physiological and molecular assays. Additionally, traditional machine learning approaches were able to identify critical visual components to predicting cell function. Thus, not only can visual information be correlated to cell function/identity, but also possible mechanisms of action can be identified using this approach. The process is fully automated, relatively inexpensive, and needs no human intervention to perform.
In summary, I show that the combination of multispectral imaging, machine learning, and CNNs are a powerful tool. This tool can not only be used to assess RPE cell function and identity in culture but also as a non-invasive release criterion for an iPSC-RPE cell based therapy to evaluate product potency, identity, and consistency.