Survey about making CyTOF, high-dimensional fluorescence flow cytometry, and RNA data publicly available
This is an informal survey to try to better understand the reasons that people do or don't share CyTOF, high-dimensional fluorescence flow cytometry (18 colors or more), or RNA (bulk RNAseq, scRNAseq, qPCR, etc) data.

This survey is completely anonymous: you will be asked for some basic demographic information, but you will not be asked for email address, name, specific institution, or any other significant identifier. Please respond for yourself: do not worry about any redundant responses with co-authors.

This survey is broken into sections. Some are focused on a particular technology, if you prefer to only answer those questions which directly apply to your research.

Demographic: Q1-Q4

CyTOF (including suspension, Hyperion/IMC, and MIBI): Q5-Q8
high-D flow (18 colors or more): Q9-Q12
RNA (bulk RNAseq, scRNAseq, qPCR, etc): Q13-Q16

General data availability and access that applies to all technologies: Q17-Q26

This survey will run from June 17 until July 31.
Q1: Where are you located?
Clear selection
Q2: What type of institution do you work at?
Clear selection
Q3: What is your professional level?
Clear selection
Q4: Do you perform CyTOF, high-dimensional fluorescence flow cytometry (18 colors or more), or RNA (bulk RNAseq, scRNAseq, qPCR, etc) assays? Please select all that apply.
********* This Section is focused on CyTOF (including suspension mass cytometry, Hyperion/IMC, and MIBI) **********
Q5. In the last 3 years (2018-2020), how many papers have you published using CyTOF (include suspension Helios, Hyperion/IMC and MIBI)? Please enter "NA" if you do not perform CyTOF assays. Please include preprint submissions. If you submitted a preprint for paper that has since been published by a journal, please count it only once.
Q6: For how many of the papers with CyTOF data did you make the raw FCS files publicly available (FlowRepository, Immport, Cytobank, Mendeley, etc)? Please enter "0" if zero datasets are publicly available, or if datasets are only available by contacting the authors. Please enter "NA" if you did not publish any CyTOF papers.
Q7: For any CyTOF paper when you *did* make your data publicly available, why did you share it? Please check all that apply.
Q8: For any CyTOF paper when you did *not* make your data publicly available, why didn't you share it? Please check all that apply.
********* This Section is focused on high-dimensional fluorescence flow cytometry (18 colors or more) *********
Q9: In the last 3 years (2018-2020), how many papers have you published using high-dimensional fluorescence flow cytometry (18 colors or more)? Please enter "NA" if you do not perform high-D flow assays. Please include preprint submissions. If you submitted a preprint for paper that has since been published by a journal, please count it only once.
Q10: For how many of the papers with high-D flow data did you make the raw FCS files publicly available (FlowRepository, Immport, Cytobank, Mendeley, etc)? Please enter "0" if zero datasets are publicly available, or if datasets are only available by contacting the authors. Please enter "NA" if you did not publish any high-D flow papers.
Q11: For any high-D flow paper when you *did* make your data publicly available, why did you share it? Please check all that apply.
Q12: For any high-D flow paper when you did *not* make your data publicly available, why didn't you share it? Please check all that apply.
********* This Section is focused on RNA (bulk RNAseq, scRNAseq, qPCR, etc) *********
Q13: In the last 3 years (2018-2020), how many papers have you published using RNA (bulk RNAseq, scRNAseq, qPCR, etc)? Please enter "NA" if you do not perform RNA assays. Please include preprint submissions. If you submitted a preprint for paper that has since been published by a journal, please count it only once.
Q14: For how many of the papers with RNA data did you make the raw files publicly available (GEO, etc)? Please enter "0" if zero datasets are publicly available, or if datasets are only available by contacting the authors. Please enter "NA" if you did not publish any RNA papers.
Q15: For any RNA paper when you *did* make your data publicly available, why did you share it? Please check all that apply.
Q16: For any RNA paper when you did *not* make your data publicly available, why didn't you share it? Please check all that apply.
********* This section is focused on general data availability and reuse *********
Q17: Have you ever reused a public dataset? Reasons might include algorithm development, or independent validation cohort. Please check all that apply.
Q18: If you have reused a public CyTOF dataset, how many times?
Clear selection
Q19: If you have reused a public high-D flow dataset, how many times?
Clear selection
Q20: If you have reused a public RNA dataset, how many times?
Clear selection
Q21: If you have not reused any public CyTOF, high-D flow, or RNA datasets, why not? Please check all that apply.
Q22: From 2018 onward, have you approached authors for datasets that were not in public repositories? For example, "data available upon reasonable request"? Please check all that apply.
Q23: From 2018 onward, have you been approached by readers for datasets that were not in public repositories? For example, "data available upon reasonable request"? Please check all that apply.
Q24: For several years, many major journals have required deposition of raw RNA data at the time of publication. What is your opinion of this?
Clear selection
Q25: If more journals started requiring public deposition of CyTOF or high-D flow datasets at the time of publication, what would you do?
Clear selection
Q26: Please include any additional and/or long-form responses here.
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