ChassiDex Data
ChassiDex is a digital information catalog of host organisms for synthetic biology, with supportive software tools.

With regard to construction of synthetic biological systems, it is observed in several iGEM projects and literature that E. coli could not express quite a number of interesting phenotypes that are native to specific organisms. In such cases it is advantageous to use those organisms and introduce synthetic circuits into them. Hence, it is desirable to have a catalogue of alternative host organisms with specific characters, and a database of protocols, parts and software tools to work with them.

Our database will consist of a collection of organisms used as hosts (or Chassis) , their physical attributes, protocols involved in culturing and transformation, and host-wise listing of biobrick parts and other vectors. It shall serve as a platform for synthetic biologists to exchange all relevant information about host organisms.

Along with the database, a set of necessary computational tools such as codon optimization will also be developed.

Contribute to our database by helping us out with this form if you are using a different host organism.

PS: You may edit the form later after submission. So the form need not be filled at one stretch.

Email address *
Name of Organism? *
Your answer
Tags
Applications
Please enter the potential uses of this organism and its special characteristics that enable these uses
Your answer
Strains
Mention the strain(s) of the Chassis you use and why *
Your answer
Life Cycle *
Please describe the life cycle of the organism. For simple life cycles, key parameters such as doubling time and optimal temperature shall be sufficient. For organisms with a complex life cycle involving stages such as sporulation or budding, please also provide a more elaborate description of stages in the life cycle.
Your answer
Culture Suppliers
In this section please provide links to culture sources from which one may obtain a culture of the organism. This may include culture collections such as ATCC, or research labs that are willing to share the organism.
Mention the Culture Suppliers *
Your answer
Maintenance Protocols
In this section, mention the storage conditions and growth mediums for maintenance of the organism. Provide links to webpages or research papers describing the full protocol. If your lab uses a customized protocol and you are willing to contribute that, you can attach it is a separate file.
Mention the Maintenance protocols *
Your answer
Transformation Protocols
In this section, mention the protocols used for transformation of organism. Provide links to webpages or research papers describing the full protocol. If your lab uses a customized protocol and you are willing to contribute that, you can attach it is a separate file.
Mention the Transformation protocols *
Your answer
Other Protocols
In this section, mention any other relevant protocols. Provide links to webpages or research papers describing the full protocol. If your lab uses a customized protocol and you are willing to contribute that, you can attach it is a separate file.
Mention Other Protocols if any
Your answer
Vectors
Please mention and provide links to cloning vectors, expression vectors, shuttle vectors, etc. that can be used with this organism.
List the Vectors *
Your answer
Parts
Please provide links to parts such as promoters, reporters, replication origin, resistance markers, etc. that can be used with this organism
List the Parts *
Your answer
Metabolic Models
Please mention and provide links to metabolic models of this organism.
List the metabolic models
Your answer
Genome Sequence
The genome sequence can be usually found in the NCBI database Please provide the link to the page which contains the genome sequence, from NCBI or any other database.
Provide a link to the Genome Sequence *
Your answer
Other relevant information
Your answer
Contributors
Please mention your contact details to be used by those who might need more help *
Your answer
References
Please add all references to the details mentioned before *
Your answer
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