We are pleased to welcome Dr. Brady Anderson at our 2024 Winter meeting!
"Offline Two-dimensional Liquid Chromatography-Mass Spectrometry for Deep Annotation of the Fecal Metabolome following Fecal Microbiota Transplant"
Biological interpretation of untargeted liquid chromatography-mass spectrometry (LC-MS) based metabolomics data depends on accurate compound identification, but current techniques fall short of identifying most features that can be detected therein. The human fecal metabolome, which is the focus of substantial recent biomedical interest, is complex, variable, incompletely annotated, and serves as an ideal matrix to evaluate novel compound identification methods. We devised an experimental strategy for compound annotation based on multidimensional chromatography and semi-automated feature alignment. Pooled fecal metabolite extract samples were fractionated using offline semi-preparative liquid chromatography. The fractions were analyzed by an orthogonal LC-MS/MS method, the resulting spectra were searched against commercial, public, and local spectral libraries, and annotations were vetted using retention time prediction. Multidimensional chromatography yielded more than a 3-fold improvement in identified compounds compared to the typical single-dimensional LC-MS/MS approach and successfully identified several rare and novel compounds, including atypical conjugated bile acid species. Most features identified by the new approach could be matched to features that were detectable but not identifiable in the original single-dimensional LC-MS data. Overall, our approach represents a powerful strategy that can be implemented with commercially available instrumentation and can be applied to any study requiring deeper annotation of the metabolome.
When:
Tuesday, January 23rd
5:30 - 7:30 PM
Where:
Boston Scientific Corporation
3 Scimed Place
Maple Grove, MN 55311
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There is no cost to attend this meeting.
