WSQ Domain 4: Biotechnology Part 2
Complete this form electronically.


1. Bring your notes to class.
2. Following your notes, write you 'HOT' questions to bring to class.
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Student ID *
Last Name *
First Name *
WATCH! As you watch the video, take notes from the slides.  Feel free to pause, rewind, rewatch as often as you like!
SUMMARY!
Please answer all questions in full sentences.
Summary Question 1 *
How does bacterial transformation work?  What do bacteria use it for?  What do genetic engineers use it for?
Summary Question 2 *
What is cDNA?  How is it made?  Why is it necessary?
Summary Question 3 *
Diagram the process of genetic engineering a bacterium. 1. Begin with a plasmid and the gene of interest.   2. Show how the gene is inserted in the plasmid. 3. Show how the plasmid is incorporated into a bacterial cell. 4. Show how the cell copies the plasmid. 5. Show how the cell expresses the gene of interest. **WRITE DONE WHEN COMPLETE**
Summary Question 4 *
Explain how reporter genes (selectable markers) can be used to separate bacteria who have taken up the transformed plasmid from those who have taken up the non-transformed plasmid.
Summary Question 5 *
Explain the relationship between single nucleotide polymorphisms (“SNPs”) and restriction fragment length polymorphisms (“RFLPs”)?  How are they caused and why do they matter?
Summary Question 6 *
Pick one real-world application that uses PCR and gel electrophoresis and specifically explain how each process is used in that application.
Summary Question 7 *
Compare and contrast therapeutic cloning with reproductive cloning.
QUESTION! Be sure to write you HOT question following you notes.  We will go over these in class during our WSQ discussion.
*big thanks to Crystal Kirch for originating this form and allowing me to use it!
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