Wastewater Surveillance for SARS-CoV-2: Method QA/QC Survey
This survey is organized by the NSF Research Coordination Network on Wastewater Surveillance for SARS-CoV-2 (https://sites.nd.edu/rcn-wastewater-sarscov2/) and is intended to gather information on standard quality control practices being performed by laboratories involved in wastewater SARS-CoV-2 monitoring.

No personally identifiable information will be collected in this survey. The results will be used to guide an upcoming workshop on QA/QC practices, troubleshooting, and interpretation.
What type of laboratory are you affiliated with?
Clear selection
What aspects of wastewater SARS-CoV-2 monitoring are you regularly involved with?
QC Terminology Definitions
For each of the questions below, the control options are defined as follows:

matrix/recovery control: a control organism spiked into raw samples at a known quantity prior to processing to evaluate overall method recovery

positive control: a reaction containing template DNA of the target to verify the assay produces a positive signal (typically used for dPCR)

standard curves: for qPCR, a dilution series of template DNA containing the assay target of interest that is evaluated to determine assay efficiency, reproducibility, and to quantify qPCR targets in experimental samples

no-template control: a PCR reaction with no template (i.e. PCR grade water) to verify lack of contamination of PCR reagents & procedure

method blank: reagent water aliquot carried through the entire sample processing procedure to verify lack of contamination of the method

inhibition control: a control to evaluate inhibition of PCR reactions evaluated by either a target spiked into sample extracts compared to the same target spiked into PCR grade water OR comparison of dilutions of sample extracts

sample replicates: a single wastewater sample split into replicate aliquots and processed separately

molecular replicates: a single extract evaluated in multiple qPCR / dPCR reactions

multiple targets: evaluating multiple SARS-CoV-2 targets using multiple assays (or multiplex assays)

endogenous wastewater control: an assay for a typical wastewater organism (e.g. PMMoV, HF183, etc) quantified to validate expected method outcomes and/or evaluate recovery
Indicate approximately how frequently your lab evaluates each control listed below. Consider primarily samples analyzed for regular surveillance program testing.
never
used during method development, but not regularly evaluated
regularly (e.g. weekly, daily, per sample, etc)
matrix/recovery control
positive control and/or standard curves
no-template control
method blank
inhibition control
sample replicates
molecular replicates
multiple targets
endogenous wastewater control
Clear selection
For the controls you currently use or have used in the past, which do you feel have provided the most reliable & useful information for interpreting your SARS-CoV-2 data?
very reliable / useful
sometimes useful
not useful
N/A
matrix/recovery control
positive control and/or standard curves
no-template control
method blank
inhibition control
sample replicates
molecular replicates
multiple targets
endogenous wastewater control
Clear selection
Briefly describe the most challenging quality control issue that you have either solved or have yet to solve.
When troubleshooting QA/QC issues, who do you typically refer to for help? (Select all that apply)
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