Background: Proteomics researchers are realizing the promise of DIA, but there is a need to develop best practices and offer opportunity for adoption. The PRG realized that there was a need for a go-to resource for new users interested in DIA studies. The need for a resource that provides information about sample processing, data acquisition and data analysis motivated the PRG to undertake this study.
Study Plan: As part of this study, the PRG has developed a prototype sample set comprised of a HeLa digest spiked with four non-human proteins at different concentrations. We will provide tested samples to participants along with recommended data-acquisition methods. Raw data will be requested from participants for PRG to analyze.
Tentative Dates:1) August 15th-November 15th: Samples were sent to requesting laboratories. Protocols available for researchers. 2) Until January 1st 2019 - Participants will deposit raw data to a MassIVE FTP account and submit information via a questionnaire about the acquired data. 3) March 2019: PRG will present the data and findings at the Annual ABRF Conference.
Updates will be available on centralized website - https://abrf.org/research-group/proteomics-research-group-prg
THE SAMPLE REQUEST PHASE FOR THIS STUDY IS OVER NOW.