Lecture 1+2

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Lecture 1+2

النادي الطلابي

1-BamHI recognizes 6 base pairs of DNA in a palindromic DNA sequence. if the first part is the sequence of given below. what's the rest of it?

1 point

A) AAC

B) TTG

C) CAA

D) GTT

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2-A researcher has identified the location of the gene that codes for protein X, and has managed to excise it from the DNA. After transcription & splicing procedures for the previously excised sequence , she generated a cDNA copy which represents the specific coding sequence for this particular protein. If she runs these two sequences through gel electrophoresis, what should she expect to see? (Note that the wells where the DNA was loaded onto the gel are at the top of the gels shown)

1 point

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3-A student was attempting to make cDNA that would code for protein Y. he began with an isolated and amplified sample of the target post-transcriptional mRNA, and all the tools required to complete the task.However, at the end of his experiment the student was left with molecules containing ribonucleotides . What most likely went wrong is:

1 point

A) The student forgot to add DNA polymerase

B) The student forgot to add DNA polymerase

C) The cloning vector already transcribed the DNA

D) The student forgot to add reverse transcriptase

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4-PCR (polymerase chain reaction) is an excellent method of generating copies of target DNA. If a single piece of double stranded DNA (dsDNA) is put into a PCR machine, how many dNA segments will there be after 3 rounds?

1 point

A) 8 segments, with the 2 original strands paired

B) 8 segments, with the 2 original strands on different segments

C) 16 segments, with the 2 original strands paired

D) 16 segments, with 2 original strands on different segments

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5- A DNA sample was sent off for Sanger sequencing. The results are shown below.What is the sequence of the template strand?

1 point

A) TGACAGTCA

B) ACTGACAGT

C)TGACTGTCA

D)ACTGTCAGT

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6-choose the incorrect statement/s regarding transcriptional regulatory sequences:

1 point

a- enhancers cannot be located within the gene itself

b- PPE harmonise the expression of genes with similar pathways

c- TATA box is in the core promoter region

D- a and c

E- all of the above are correct

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7-‘’Scientists took advantage of LUCIFERase same as GFP’’, choose the correct statement/s

1 point

a- it gives us how active certain regions of the promoter are

b- we use its own promoter in this experiment

c- if the repressor region is removed there would be a large increase in LUCIFERase relative activity

d- a and c

e- all of the above

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8-we use reverse transcriptase in:

1 point

a- cDNA library that is made by mRNA without introns

b- cDNA library that is made by mRNA with included introns

c- Genomic library

d- a and b

e- none of the above

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9- Which of the following statements best describes Y2H system ?

1 point

a- it is a molecular technique to study what regions are important for the transcription to happen

b- blue colonies indicate the presence of only activating (or binding) domain of GAL4

c- if X and Y protein genes are recombinant with AD and BD respectively, blue colonies indicate that they interact with each other

d- a and c

e- none of the above is true

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