FINAL Exam || Biochemistry Lab 
يوفر لكم فريق نشامى الصيدلة بنك الأسئلة الالكتروني الأول من نوعه :
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Complete hydrolysis of proteins gives:
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Biuret test is general test identifies peptide bonds of any polypeptide and protein by using :
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Biuret test detect : *
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What is the positive color of proline with ninhydrin test? *
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Millon’s test is specific test identifies tyrosine interaction with Hg+2 in alkaline solution, it gives positive brick red color with tyrosine amino acid and proteins contain tyrosine.  
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Millon’s test gives false positive result with :  *
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Which of the following chemical tests detect tryptophan? *
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Which of the following compounds does not give a positive result with lead acetate test? *
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Precipitation of therapeutic proteins (e.g. insulin) in formulations is very important to ensure their activities.  
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Every protein has minimum solubility at a pH value called isoelectric point at which it has a net charge of zero.  
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The isoelectric point of proteins is around  *
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At acidic pH protein molecules will have a negative charge and in alkaline solutions proteins will have a  positive charge.  
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At low concentrations of salts, solubility of proteins increases is called :
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Low concentrations of neutral salt ions increases solubility and they do affect the isoelectric point  
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Heavy metal ions (Zn2+ , Fe3+ , Cu2+ , Hg+2 , Pb+2 , Cd2+ , Ag+1 , ….) inter-chelate with proteins causing their ...............  
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Tannic acid and picric acid are examples of what?
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The attractive force between charged atoms of proteins ............... with the addition of solvents with low D.E  
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Which of the following solutions would you expect to have the least precipitation?   *
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Which of the following salts would give the highest precipitation with proteins? *
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Enzymes are generally protein molecules that act as biological catalysts. They increase the rate of the chemical reactions without being consumed in the overall reaction.  
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Enzyme activity can be assessed by observing:  
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Catalase detoxifies hydrogen peroxide (H2O2 ) by converting it to  
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Catalase is the trivial name of the enzyme. The systemic name is H2O2 - H2O2 oxidoreductase.    *
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Catalase catalyzes the detoxification of H2O2 using :
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In the peroxidative type, catalase catalyzes a reaction where one molecule of H2O2 acts as a donor of hydrogen, and another molecule of H2O2 acts as an acceptor of hydrogen.
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Which of the following molecule serves as a hydrogen donor in the peroxidative type of catalase reaction 
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Catalase is .......... efficient in catalysing the peroxidative type compared to the catalytic type.  
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The optimum temperature of the enzyme. For the enzymes in the human body is 
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The optimum pH is the same for different enzymes, since it depends on its structure and where it should work  
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the green X refers to what? *
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Captionless Image
acylglycerols and phosphoglycerides are  
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  The hydrocarbon chain CH3(CH2)n in fatty acids may be unsaturated (have no double bonds) or saturated (have one or more double bonds)  
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Olive oil is partially miscible with  *
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The higher the dielectric constant, the higher is the polarity of the solvent
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the hydrolysis of esters of fatty acids and glycerol in TAG molecules under alkaline conditions (NaOH or KOH) to produce salts of fatty acids and free glycerol  
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Saponification is carried out by using aqueous KOH, not alcoholic KOH, because using beef tallow or any other fat would be insoluble in water and this leads to slowing the reaction which occurs only upon boiling  
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The addition of HCl to the soap results in converting fatty acids back to their free form (protonated, unionized) leading to their precipitation. The resulting free fatty acids precipitate in the form of scum  
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soaps are potassium or sodium salts of long alkyl chains ending with sulfonate groups *
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what is the chemical test used to distinguish between saturated and unsaturated fatty acids and for determination the degree of unsaturation of fatty acids.
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The greater degree of unsaturation (more double bonds) the more halogen is taken up, so the less intense the color of the solution.  
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