PREANALYTICAL ASPECTS OF LABORATORY TESTING

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HISTOPATHOLOGY MODULE

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What is the recommended amount/ratio of formalin (fixative) to tissue?

1 point

1:5

As much as the container permits

10:1

1:10

Equal to the weight of the specimen

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What is the goal of fixation?

1 point

Prevent putrefaction

Prevent osmotic swelling of cells

Render the tissue suitable for subsequent staining

Harden the tissue for easier handling

All of the above

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What information is required on the specimen bottle? (Tick all that is required)

3 points

Tick here

Patient's name

Patient's contact number

Patient's address

Doctor's contact number

Hospital Registration Number (RN)

Type of specimen

Which of the following are TRUE?

3 points

Tick here

Dextrose 5% is an acceptable substitute for formalin

Specimens for HPE should be sent in an adequately sized container that does not distort/compress the specimen

Frozen sections should be sent in a dry container filled without any fixative

Specimens for Immunofluorescence studies should be sent in a container filled with Phosphate buffered Saline

For uncommon requests and queries regarding HPE /Cytology / Frozen Sections; what is the BEST way to address the issue?

1 point

Call the on-call Microbiologist

Ask for help on Social Media

Read the lab handbook, and call the Anatomic pathologist on-call if the handbook is unable to resolve the issue

Ask a senior for help

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