iGEM Quiz - Synthetic Biology Principles

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This MCQ tests Chapter 3 of the iGEM guidebook, which can be found here:
http://igem.org/wiki/images/7/71/AITF_iGEMHS_Guidebook_Final.pdf

Good luck!

- The CLS/CLSG iGEM Team

Email *

What is the name of the enzyme that can stick free DNA ends? *

2 points

Name (optional):

What are the three steps in the 3A assembly method? *

1 point

Restriction site transformation, PCR Assembly, Restriction digestion

PCR Assembly, Transcription, Restriction digestion

Restriction digestion, ligation, transformation

Transcription, Translation, PCR Assembly

Tick the promoters that are controllable *

3 points

araBAD

IPTG (Isopropyl beta-D-thiogalactoside)

Amylase

LacI

Adh-1

Required

What is the function of a terminator? (SELECT ALL THAT ARE TRUE) *

2 points

It blocks the progress of DNA Polymerase

It blocks the progress of RNA Polymerase

It terminates translation

Required

What is the inducing agent for a T7 promoter *

1 point

T7 RNA Polymerase

IPTG (Isopropyl beta-D-thiogalactoside)

Lactose

T7 Promotion Ligase

Complete the sentence: The sequence of nucleotides in a ___ causes the DNA at the end of a gene to fold. *

1 point

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