MCW Chemical Library NMR Screen - Protein Candidate Pre-Submission Form
Basic Information
1) Principal Investigator Name:
Your answer
2) Name of person submitting protein:
Your answer
3) Phone number:
Your answer
4) Protein name (including residue range of submitted protein):
Your answer
5) Exact sequence of submitted protein (including affinity tags, amino acids left after protease cleavage, post-translational modifications, etc.):
Your answer
6) Protein Database ID (Uniprot, NCBI, etc.):
Your answer
7) Molecular Weight (kDa):
Your answer
8) If you are screening multiple proteins, is this target your top priority?
Protein Expression
9) Have you ever expressed and isolated this protein before?
If YES to question 9
10) Typical yield (mg/L) in rich media:
Your answer
11) Typical yield (mg/L) in minimal media:
Your answer
12) Preferred buffer conditions:
Your answer
13) Buffer conditions NOT tolerated:
Your answer
14) Molar extinction coefficient (ε280 nm):
Your answer
15) Estimated maximum protein solubility (µM or mg/mL):
Your answer
Note: Default storage is at 4°C and default experimental temp is 25°C. If this is not tolerated, indicate preferred storage conditions below:
Your answer
Sub-Cloning of Protein
16) Is your protein sub-cloned into an expression vector?
If YES to question 16:
17) What expression species?
18) What vector (e.g. pET 28b, pQE 80, pGEX 6P-1)?
Your answer
If NO to question 16:
19) Do you need plasmids for bacterial expression?
Structural Information
Structure known?
Sequence of submitted protein identical to structure?
PDB accession number(s) (NMR structures preferred if known):
Your answer
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