DIB causes of concern

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These are the most common things that go wrong when you can't form bilayers anymore:

Lipids

Agarose

Hexadecane (oil)

Dirty device/incubation chamber

Buffers (solutions in general)

Dirty coverslips (plasma cleaner)

MilliQ (filter needs changing?)

Nitrogen (is it wet/contaminated?)

Pipettes contaminated

Silicone oil

Other:

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