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Testing NgAgo genome-editing system
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Have you tested CRISPR-Cas9 and NgAgo system head-to-head?
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Who wins: CRISPR-Cas9 or NgAgo system?
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Do you see indels with NgAgo system?
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Do you see epitope tag knockin with NgAgo system?
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How happy are you with the NgAgo system?
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Final verdict
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Protocol: NgAgo system
Do you phosphorylate ssDNA guide in house or get it synthesised from a company?
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Do you use gel-purified (PAGE) ssDNA guides?
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What is the main goal of your NgAgo experiments?
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What method do you use for NgAgo delivery into cells?
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How much 5'phospho ssDNA guide do you use for each transfection?
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How much ssODN (ultramer) do you use for each transfection?
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How much plasmid DNA (NgAgo) do you use for each transfection?
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Any tips on 5'-phospho ssODN? Design, purification, amount etc
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