BioMakespace Autoclave Training

JavaScript isn't enabled in your browser, so this file can't be opened. Enable and reload.

BioMakespace Autoclave Training - Quiz Questions

Welcome! This is a quiz to confirm you understand how to use the autoclave safely. You need to pass the quiz with 100% pass rate. Once you've passed this quiz, in the addition to the online training and quizes of BioMakespace Safety training part I and part II, you will need to book an in person safety assessment during which you will be also asked to demonstrate your knowledge of using the autoclave.

Email *

Name *

Your name as on your Makespace membership

Membership number *

Your Makespace membership number. If you have not been issued with a membership number, please contact admin@makespace.org to get yours. Thank you.

What do we use the autoclave for? Tick all that apply *

for sterilizing media

for sterilizing reagents

for sterilizing instruments

for sterilizing biological waste (including Class 1GM organisms) so it can be disposed of safely

Required

Where do you find the autoclave in BioMakespace? *

In the main Biolab

In the middle Biolab

In the maker lab at BioMakespace

In the ideaspace kitchen

What are the three highest risks of using the autoclave in Biomakespace? *

High pressure - explosion, rapid release of gas or steam

Radiation - burns and exposure

High temperatures - burns and scalds

Chemical exposure - burns and respiratory problems

Manual handling - fetching loads in and out, carrying water

Exposure to infectious pathogens

Other:

Required

What personal protective equipment (PPE) must you wear when using the autoclave? *

A lab coat

Safety goggles

Hard hat

Closed toe footwear

Heat proof gloves

All of the above

Required

What validation testing needs to be done by you when using the autoclave? *

Each item/bag should have autoclave indicator tape to show it has been autoclaved

A TST (temperature, steam, time) validation strip should be added to the centre of each load before running the autoclave

Each load should be checked for sterility when it is removed from the autoclave

Print out a report of the autoclave run

Required

What type of water is used to fill the autoclave? *

100% tap water

100% deionised water

80/20 tap water and deionised water

20/80 tap water and deionised water

50/50 tap water and deionised water

Where should the autoclave probe be when you start a run? *

The probe must sit inside the load, including inside the bag or BioBin

The probe must be attached at the top of the inside of the autoclave to measure the highest temperature inside

The probe must sit near the centre of the load but does not need to be inserted into the bag or BioBin

What should you see once the door latch and bolt are closed correctly? *

The “Thermal Lock” light illuminates

the “Door Closed” light illuminates on the panel

The “Heat On” light illuminates

all the above

When does the thermal lock disengage? *

immediately after the autoclave run has finished

when the temperature of the load reaches 80 degrees celcius

when you start rotating the bolt anti-clockwise

What should you always wear when removing a load from the autoclave? *

protective heat resistant /silicone gloves

safety goggles

both

Which of these steps do you do when disposing of autoclaved waste? *

put the autoclaved biological waste in a thick black bag and discard as normal waste

put autoclaved biological waste in a thick black bag and then in a yellow and black "tiger stripe" bag and then in the yellow bins in the bin store (outdoor bin store) marked "yellow bags"

put autoclaved biological waste in a thick black bag and then in a yellow and black "tiger stripe" bag and then in the normal bin

What should you do if there's a pressure vessel breach? *

run out as quickly as you can and bring yourself to the evacuation point

try and deal with it yourself as you want to re-run the autoclave so you have sterile media and reagents to continue your experiments

shout for help and break the fire alarm point glass, and wait till the building facilities manager arrives

shout for help and break the fire alarm point glass, then follow the fire evacuation procedure.

Other:

Which of the following steps should you take if there's a microbiological spillage? Tick all that apply *

Immediately remove any contaminated clothing and other protective equipment and wash affected areas with soap and water.

Alert other lab members of spillage.

Decontaminate the spillage and area with 10% bleach, following the usual spillage procedures

Mop up straight away with tissues and dispose of them in a clear bag for autoclaving

Decontaminate the spillage and area with Chemgene, following the usual spillage procedures.

Required

How long does it roughly take for the autoclave to run? *

15 minutes

2 hours

over night

Which of the following materials should not be autoclaved? Tick all that apply *

Agar

Bleach

Ethanol

Mutagens, teratogens, carcinogens

Polypropylene

Required

Where should you stand when opening the autoclave? *

In front of the door

On the same side of the autoclave that the door opens

On the side the door hinges are fixed, keeping out of direct line of the door when you open it

What should you do if the autoclave run fails and the TST indicator is still yellow? *

Remove the items and treat as non-sterilised, leave in boxes by autoclave then contact the safety team by email

Leave all the items in the autoclave then call the Biomakespace emergency number

Try to chemically disinfect all of the items then contact the safety team by email

How should you prepare an autoclave bag to be autoclaved? Tick all that apply *

Ensure it is only 1/2 full

Ensure it is only 2/3 full

Tightly gather the top of the bag with autoclave tape

Loosely gather the top of the bag with autoclave tape, leaving at least a 1 inch hole

Fold over the top of the bag and seal with autoclave tape

Split petri dishes to no more than 20 per bag

Look out for points (e.g. pipette tips) and double bag if these have been incorrectly discarded in the autoclave bags

Required

Submit

Clear form

Never submit passwords through Google Forms.

This form was created inside of makespace.org. Report Abuse

Forms