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RNA-Sequence Data Description Form
Use this form to describe RNA-sequence data provided for loading into EuPathDB databases.
What is the title of your experiment?
The title can be as long as a sentence and should provide a clear summation of the experiment
Your answer
Provide a brief description of this data
When people see this data on EuPathDB sites, how would you like to describe it to them?
Your answer
Provide the name and contact info for the Primary investigator(s)
Provide a name, address, phone number and email for each. (e.g. Principal Investigator and the person(s) who prepared samples and/or analyzed the data.
Your answer
Provide the name and contact info for the primary author.
This will be used as a part of the experiment title ie. Your RNA-seq experiment (first author et al.)
Your answer
Provide the name(s) and contact info for any collaborators
This would be anyone else that should be mentioned in the dataset description for this experiment
Your answer
Sample Preparation
What is the name of the organism that was the source of the sample(s)
Please use full scientific name and strain, ie. Toxoplasma gondii ME49
Your answer
What were the samples (source of RNA) that were used?
Are they related in anyway (did they come from the same culture, were they used for technical replicates)?
Your answer
What experimental factors varied in this experiment?
ie. what were the interesting differences in your sample groups
Your answer
What were the protocols or kits used in this experiment?
ie. any highlights that would be important to include with your data
Your answer
Were strand specific libraries made?
For strand specific libraries please indicate the extension of the forward strand and the one for the reverse strand
Your answer
Sequencing
What is the name of the sequencing method (solexa, 454, etc.)?
Your answer
Was this a paired-end sequencing run?
What software was used for your analysis?
Your answer
Results
Has this data been submitted to a sequence archive - ie. SRA? If yes, provide the accession number(s). If not, list the data files that were generated and indicate how they relate to the samples described above
If files have been submitted directly, please indicate which strand each file belongs to if these was strand specific sequencing
Your answer
Which genome should this data be aligned to?
Please use full scientific name and strain, ie. Toxoplasma gondii ME49
Your answer
Is this data associated with a Publication?
ie. citation, PubMed ID or DOI, etc....
Your answer
Other infomation
Provide any additional information that is important for understanding this data and how to analyze it
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