Westmead Electron Microscopy - Vitrobot Training Competency

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Laboratory basic safety

*Required

Email address *

Name *

The Vitrobot is in a PC2 facility. Tick all that apply. *

1 point

Washing hands before leaving the laboratory is a requirement

Food and drink can be brought into the PC2 laboratory

Knowing what to do in an emergency is a requirement for using the laboratory

I need to have been signed off on PC2 training before using the Vitrobot

Required

The Vitrobot can be used outside normal laboratory operating hours of 8:30-17:00? *

1 point

True

False

When using the Vitrobot, I am allowed to have a mobile phone in the PC2 lab? *

1 point

True

False

You are in the PC2 lab and the oxygen alarm sounds indicating the oxygen level is low. Tick all that apply *

1 point

Reset the oxygen alarm panel

Stop what I am doing and leave the PC2 lab immediately

Continue on with my experiment and hope that a staff member checks that I am ok

Check oxyegn level returns to normal before returning to the PC2 lab

Required

The Vitrobot can be used if no one else is in the Electron Microscope Facility *

1 point

True

False

What type of water is used in the humidifier of the Vitrobot *

1 point

Tap water

Distilled water

MilliQ water

You need to wear latex or nitrile gloves when liquifying ethane with liquid nitrogen *

1 point

True

False

You should replace blotting paper on the Vitrobot (Tick all that apply) *

1 point

when the blotting paper looks wrinkled

every time you blot a sample

at the start of a new Vitrobot session

when the Vitrobot tells you to change the paper

When loading the tweezers into the specimen arm of the Vitrobot, it doesn't matter what orientation they are in? *

1 point

True

False

If there is excess liquid ethane at the end of the Vitrobot session, you *

1 point

Dispose of it down the fumehood sink

Let the ethane evaporate in the fumehood

Decant the ethane into a labelled waste bottle

Store the ethane to use again in the next Vitrobot experiment

Before shutting down the Vitrobot, tweezers need to be removed from the plunging device *

1 point

True

False

To call police, fire, or ambulance from inside ICPMR, I need to dial *

1 point

0000

000

2222

911

You need to be familiar with the SOP, SWP, emergency contacts, as well as any other safety policies in place before accessing the Vitrobot? *

1 point

True

False

If the ethane cylinder is empty, I can replace the empty cylinder with a full ethane cylinder myself? *

1 point

True

False

Once specimens have been frozen on the Vitrobot they can be stored in liquid nitrogen and viewed on the TEM on another day *

1 point

True

False

Before shutting down the Vitrobot you need to (Tick all that apply) *

1 point

Remove the tweezers

Remove the liquid ethane container

Remove the blotting papers

Required

The spindle needs to be removed from the liquid nitrogen/ethane container before loading onto the Vitrobot *

1 point

True

False

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