A short (<2 min) survey for microscope users
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1. What is your background (check all that apply)?
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2. For how long have you been using a microscope?
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3. How often do you use a microscope?
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4. How many images do you acquire in a typical session at the microscope?
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5. Do you make quantitative measurements from microscopic images?
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6. If yes, approximately what percentage of images you acquire is used for quantitative analysis?
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7. Are you familiar with the term "flat-field correction"?
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8. Do you believe such image distortions affect quantitative analyses performed in your laboratory?
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9. As a percentage of the actual image intensity, how much would you estimate such distortions affect images collected in your laboratory?
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10. The image below shows an Alexa Fluor 680 stained medium containing yeast cells, which appear as dark blobs. How will the uneven illumination in this image will affect a standard automatic cell counting algorithm (Otsu's method)?
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11. How do you think the uneven illumination will affect segmentation accuracy in the above image (using Otsu's method)?
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12. The image below shows HELA cells from a high content screen, with GFP-stained cytoplasm. How will the uneven illumination will affect a standard automatic estimate of cell size (using Otsu's method)?
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13. How do you think the uneven illumination will affect a standard automatic estimate of cell GFP intensity in the previous image?
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14. Which flat-field correction method(s) do you use (check all that apply)?
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15. How often do you perform flat-field correction on images you've personally acquired?
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16. For which type(s) of imagery (if any) do you believe flat-field correction is UNNECESSARY?
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17. Would you be interested in using a flat field correction method that does not require the acquisition of any special calibration images, but performs similar to or better than those methods?
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