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Plasmids and virus services are now available through Addgene (https://www.addgene.org/Viviana_Gradinaru , https://www.addgene.org/viral-service/aav-prep/ ) .

We are not a core facility but rather produce viruses in collaborative mode depending on bandwidth and funds available. If this works for your projects please contact the PI, Viviana Gradinaru, at viviana@caltech.edu to discuss.

To request published reagents which are not on Addgene, please fill out this form for the plasmid(s) or virus(es) you are requesting.

If requesting AAV-PHP rep-cap virus, note that an MTA for AAV9 from U Penn Vector core is required. This must be signed by both U Penn and your institution and sent by electronic copy to Caltech's Technology Transfer office (mta@caltech.edu) before Caltech will issue an MTA.

The UPenn MTA form may be found at: https://clover.caltech.edu/documents/17-mta-pennwilson-omnibus_academic_v3f.pdf Please initiate this as soon as possible.

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PI/Group name *
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Request- Published plasmids. We produce viruses in collaborative mode depending on bandwidth and funds available. If this works for your projects please contact the PI, Viviana Gradinaru, at viviana@caltech.edu to discuss. *
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In one or two sentences, describe what you intend to use this reagent for. *
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The following plasmids are available on Addgene.org (https://www.addgene.org/Viviana_Gradinaru/). Many AAV-PHP.eB and PHP.S viruses are distributed by Addgene (https://www.addgene.org/viral-service/aav-prep/)
AAV-PHP.B rep-cap plasmid (Addgene ID: 103002)
AAV-PHP.B2 rep-cap (Addgene ID: 103003)
AAV-PHP.B3 rep-cap plasmid (Addgene ID: 103004)
AAV-PHP.eB rep-cap plasmid (Addgene ID: 103005)
AAV-PHP.S rep-cap plasmid (Addgene ID: 103006)

pAAV-CAG-tTA - universal VAST inducer (Addgene ID: 99118)
pAAV-hSyn1-tTA - neuron restricted VAST inducer (Addgene ID: 99119)
pAAV-ihSyn1-tTA - neuron restricted VAST inducer with positive feedback (Addgene ID: 99120)
pAAV-ihSyn1-DIO-tTA (Addgene ID: 99121)

pAAV-mDlx-NLS-mRuby2 (Addgene ID: 99130)
pAAV-mTH-GFP (Addgene ID: 99128)

pAAV-CAG-mRuby2 (Addgene ID: 99123)
pAAV-CAG-mTurquoise2 (Addgene ID: 99122)
pAAV-CAG-EYFP (Addgene ID: 104055)

pAAV-CAG-DIO-mTurquoise2 (Addgene ID: 104059)
pAAV-CAG-DIO-mRuby2 (Addgene ID: 104058)
pAAV-CAG-DIO-EYFP (Addgene ID: 104052)

pAAV-hSyn1-mRuby2 (Addgene ID: 99126)
pAAV-hSyn1-mTurquoise2 (Addgene ID: 99125)

pAAV-TRE-mRuby2 (Addgene ID: 99114)
pAAV-TRE-mTurquoise2 (Addgene ID: 99113)
pAAV-TRE-eYFP (Addgene ID: 104056)

pAAV-TRE-DIO-mRuby2 (Addgene ID: 99117)
pAAV-TRE-DIO-tdTomato (Addgene ID: 99116)
pAAV-TRE-DIO-mTurquoise2 (Addgene (ID: 99115)

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We ship reagents by FEDEX. Please provide a FedEx account number below
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If you are requesting a plasmid that uses the GFAP (GfABC1D) promoter, please note that this plasmid was developed in the laboratory of Michael Brenner at UAB. No further MTAs are required, but by checking this box you agree that you will cite and acknowledge their paper http://onlinelibrary.wiley.com/doi/10.1002/glia.20622/abstract in any publications arising from the use of this plasmid.
Note: AAV-PHP.A, -PHP.B, -PHP.S and -PHP.eB are modifications of AAV9, which we received from the University of Pennsylvania. Under our MTA with Penn, we are required to ensure that all requesters of our vectors also have an MTA with Penn for AAV9. Please provide a copy of that MTA to us. If you don’t yet have it, you can obtain it by downloading it from the following link and following the instructions on the cover page. https://www.med.upenn.edu/gtp/vectorcore/user_documents/MTA-PennWilson_Omnibus-AcademicV2.pdf
For questions about AAVs or virus production, please contact Viviana Gradinaru (viviana@caltech.edu)
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