Section 1 of 15
MGEF embryo culture, cryopreservation and IVF media / kit requests
MGEF has standardized its own quality controlled embryo culture medias, embryo / sperm cryopreservation and recovery medias as well its own IVF medias.
each batch of media is QCed for fertlisation / embryo survival and embryo development & progression inVitro culture till blastocyst stage.
please place your media requests at least 3 weeks in advance and for a maximum of 20-40ml per media at a time.
All media compositions and detailed protocols can be requested to mgef@ncbs.res.in.
each batch of media is QCed for fertlisation / embryo survival and embryo development & progression inVitro culture till blastocyst stage.
please place your media requests at least 3 weeks in advance and for a maximum of 20-40ml per media at a time.
All media compositions and detailed protocols can be requested to mgef@ncbs.res.in.
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Section 2 of 15
MGEF embryo culture, cryopreservation and IVF media / kit requests
MGEF has standardized its own quality controlled embryo culture medias, embryo / sperm cryopreservation and recovery medias as well its own IVF medias.
each batch of media is QCed for fertlisation / embryo survival and embryo development & progression inVitro culture till blastocyst stage.
please place your media requests at least 3 weeks in advance and for a maximum of 20-40ml per media at a time.
All media compositions and detailed protocols can be requested to mgef@ncbs.res.in.
each batch of media is QCed for fertlisation / embryo survival and embryo development & progression inVitro culture till blastocyst stage.
please place your media requests at least 3 weeks in advance and for a maximum of 20-40ml per media at a time.
All media compositions and detailed protocols can be requested to mgef@ncbs.res.in.
Section 3 of 15
M2 media
MGEF-M2 media is a buffered embryo handling and maintenance media.
M2 is provided as single use 4ml aliquots which can be stored at -30C for up to one year.
Each aliquot should be fully thawed, mixed and filtered prior each use.
MGEF can provide maximum 40 ml of M2 media at a time.
M2 is provided as single use 4ml aliquots which can be stored at -30C for up to one year.
Each aliquot should be fully thawed, mixed and filtered prior each use.
MGEF can provide maximum 40 ml of M2 media at a time.
Number of M2 4ml aliquots requested
Number of M2 4ml aliquots requested
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Section 4 of 15
KSOM media
MGEF-KSOM media is an embryo culture media. It is provided as single use 4ml aliquots which can be stored at -30C for up to one year.
Each aliquot should be fully thawed, mixed, filtered and fully equilibrated at 37C / 5% C02 prior each use.
MGEF can provide maximum 40 ml of KSOM media at a time.
Each aliquot should be fully thawed, mixed, filtered and fully equilibrated at 37C / 5% C02 prior each use.
MGEF can provide maximum 40 ml of KSOM media at a time.
Number of KSOM 4ml aliquots requested
Number of KSOM 4ml aliquots requested
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Section 5 of 15
Sucrose-PB1
Sucrose-PB1 is a buffered 0.25M sucrose solution required for embryo Cryorecovery.
MGEF Sucrose-PB1 is provided as 1ml single Use aliquots which can be stored at 4C for up to 3 months.
MGEF can provide maximum 20 ml of Sucrose-PB1 media at a time.
MGEF Sucrose-PB1 is provided as 1ml single Use aliquots which can be stored at 4C for up to 3 months.
MGEF can provide maximum 20 ml of Sucrose-PB1 media at a time.
Number of Sucrose-PB1 1ml aliquots requested
Number of Sucrose-PB1 1ml aliquots requested
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ZERO = Sucrose-PB1 not required
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Section 6 of 15
HTF media
MGEF-HTF is a high calcium HTF Media used in mouse inVItro Fertilisation assays.
HTF is provided as 4ml single Use aliquots which can be stored at 4C for 3 months.
Reduced glutathione (GSH) solution is also required fo some IVF procedures. GSH solution can be provided as 50ul aliquots. GSH is quite unstable and should be stored at -80C for a maximum of 3 months.
HTF is provided as 4ml single Use aliquots which can be stored at 4C for 3 months.
Reduced glutathione (GSH) solution is also required fo some IVF procedures. GSH solution can be provided as 50ul aliquots. GSH is quite unstable and should be stored at -80C for a maximum of 3 months.
Number of HTF 4ml aliquots requested
Number of HTF 4ml aliquots requested
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Number of GSH 50ul aliquots requested
Number of GSH 50ul aliquots requested
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Section 7 of 15
SPI media
Sperm Pre-Incubation Media is required during IVF procedures.
SPI is provided as 1ml single use aliquots which can be stored at 4C for up to 3 months.
SPI is provided as 1ml single use aliquots which can be stored at 4C for up to 3 months.
Number of SPI 1ml aliquots requested
Number of SPI 1ml aliquots requested
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Section 8 of 15
gCPA media
This is a Sperm Cryo Protetantant Agent containing L-glutamine used in mouse Sperm Cryopreservation Procedures.
gCPA is provided as 1ml single use aliquots to be stored at Room Temperature, protected from light, for up to 3 months maximum.
gCPA is provided as 1ml single use aliquots to be stored at Room Temperature, protected from light, for up to 3 months maximum.
Number of gCPA 1ml aliquots requested
Number of gCPA 1ml aliquots requested
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Section 9 of 15
DMSO/PB1 solution
1M DMSO/PB1 solution is required in mouse embryo vitrification procedures.
DMSO/PB1 is provided as 1ml aliquots which can be stored at 4C for up to 3 months.
DMSO/PB1 is provided as 1ml aliquots which can be stored at 4C for up to 3 months.
Number of DMSO/PB1 1ml aliquots requested
Number of DMSO/PB1 1ml aliquots requested
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ZERO = DMSO/PB1 not required
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Section 10 of 15
DAP213 solution
DAP213 is required for mouse embryo vitrification procedures.
MGEF DAP213 is provided as 1ml (=500ul of SolutionA +500ul of Solution B) aliquots which can be stored at 4C for up to 3 months.
MGEF DAP213 is provided as 1ml (=500ul of SolutionA +500ul of Solution B) aliquots which can be stored at 4C for up to 3 months.
Number of DAP213 1ml aliquots requested
Number of DAP213 1ml aliquots requested
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Section 11 of 15
Embryo Handling pipette Kit
the KIT contains 5 MGEF hand pulled and forged borosilicate glass mouse embryo handling pipettes + 1 silicon tubing + 1 silicon washer
Number of embryo handling pipette Kits requested
Number of embryo handling pipette Kits requested
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1 kit
4
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Section 12 of 15
mouse Embryo infundibulum Transfer pipette Kit
the KIT contains 5 MGEF hand pulled and forged borosilicate glass mouse embryo oviduct transfer pipettes + 1 silicon tubing + 1 silicon washer. Pipettes are specially calibrated for infundibulum transfers of embryos.
Number of embryo Transfer pipette Kits requested
Number of embryo Transfer pipette Kits requested
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Section 13 of 15
Sample Pick-Up logistics
Do tell us the approximative date of when you would want to pick up the samples.
Please note that MGEF will need aminimum of 2-3 weeks to process your order.
Transport containers and shipment logistics will need to be provided and organised directly by the requestor.
Please note that MGEF will need aminimum of 2-3 weeks to process your order.
Transport containers and shipment logistics will need to be provided and organised directly by the requestor.
Approximative requested Sample Pick-UP Date / week? 1 month
Approximative requested Sample Pick-UP Date / week? 1 month
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Section 14 of 15
Terms and Conditions
I, hereby acknowledge and agree to the following terms and conditions associated with the use of services provided by the NCBS/inStem Mouse Genome Engineering Facility:
- Acknowledgment in Publications: All publications, presentations, posters, theses, or other dissemination materials that include work supported by or related to services provided by the NCBS/inStem MGEF must acknowledge the facility. Recommended Acknowledgment Statement: "The authors must acknowledge the technical support in mouse-assisted reproductive services provided by Mouse Genome Engineering Facility (MGEF), at the National Centre for Biological Sciences in Bangalore, and the Institute for Stem Cell Science and Regenerative Medicine, Bangalore.
- Best-Effort Basis: All services are provided on a best-effort basis. While the facility endeavours to deliver high-quality work and successful outcomes, the nature of genome engineering and related services entails inherent technical uncertainties. Therefore, success cannot be guaranteed.
- No Refund Policy: The facility operates under a no-refund policy. Regardless of the outcome or success of the project, no financial reimbursement shall be made for any project that does not result in the desired technical or scientific result.
Signature of Requestor/Investigator: _M. Arun kumar____________________
Date: 24/04/2026______
Section 15 of 15
Signature/DATE
Vishal 23/06/2026
Name of person Submitting this form:
Name of person Submitting this form:
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Vishal Vyas
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23/06/226
23/06/226
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